

@article {978,
	title = {Identification of a Novel Human Polyomavirus in Organs of the Gastrointestinal Tract},
	journal = {PLoS ONE},
	volume = {8},
	number = {3},
	year = {2013},
	abstract = {<p>Polyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using a generic polyomavirus PCR targeting the VP1 major structural protein gene, a novel polyomavirus was initially identified in resected human liver tissue and provisionally named Human Polyomavirus 12 (HPyV12). Its 5033 bp genome is predicted to encode large and small T antigens and the 3 structural proteins VP1, VP2 and VP3. Phylogenetic analyses did not reveal a close relationship to any known human or animal polyomavirus. Investigation of organs, body fluids and excretions of diseased individuals and healthy subjects with both HPyV12-specific nested PCR and quantitative real-time PCR revealed additional virus-positive samples of resected liver, cecum and rectum tissues and a positive fecal sample. A capsomer-based IgG ELISA was established using the major capsid protein VP1 of HPyV12. Seroprevalences of 23\% and 17\%, respectively, were determined in sera from healthy adults and adolescents and a pediatric group of children. These data indicate that the virus naturally infects humans and that primary infection may already occur in childhood.</p>
},
	isbn = {1932-6203},
	issn = {19326203},
	doi = {10.1371/journal.pone.0058021},
	author = {Korup, Sarah and Rietscher, Janita and Calvignac-Spencer, S{\'e}bastien and Trusch, Franziska and Hofmann, J{\"o}rg and Moens, Ugo and Sauer, Igor and Voigt, Sebastian and Schmuck, Rosa and Ehlers, Bernhard}
}
