

@article {9102,
	title = {{Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms}},
	journal = {Journal of Applied Microbiology},
	volume = {111},
	number = {5},
	year = {2011},
	pages = {1159{\textendash}1175},
	abstract = {AIMS: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. METHODS AND RESULTS: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20 degrees C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10.5, 8.1 and 17.2 days, respectively. CONCLUSIONS: Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters. SIGNIFICANCE AND IMPACT OF THE STUDY: The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters.},
	keywords = {Lactobacillus amylovorus, Microbial source tracking, Oxygen, Persistence, Pig-associated Bacteroidales markers, Quantitative real-time PCR, River water, Temperature},
	isbn = {1365-2672 (Electronic){\$}\backslash{\$}r1364-5072 (Linking)},
	issn = {13645072},
	doi = {10.1111/j.1365-2672.2011.05131.x},
	author = {Marti, R and Mieszkin, S and Solecki, O and Pourcher, A M and Hervio-Heath, D and Gourmelon, M}
}
