

@article {830,
	title = {Rotavirus seasonality in urban sewage from Argentina: effect of meteorological variables on the viral load and the genetic diversity.},
	journal = {Environmental research},
	volume = {138},
	year = {2015},
	pages = {409-415},
	abstract = {In Argentina, the rotavirus disease exhibits seasonal variations, being most prevalent in the fall and winter months. To deepen the understanding of rotavirus seasonality in our community, the influence of meteorological factors on the rotavirus load and the genetic diversity in urban raw sewage from {C\&\#xF3;rdoba} city, Argentina were evaluated. Wastewater samples were collected monthly during a three-year study period and viral particles were concentrated by polyethylene glycol precipitation. {RT-nested} {PCR} was applied for rotavirus detection, and {VP7/VP4} characterization and real-time {PCR} for rotavirus quantification. Both molecular techniques showed relatively similar sensitivity rates and revealed rotavirus presence in urban wastewater in cold and warm seasons, indicating its circulation in the local community all year round. However, a slight trend for rotavirus circulation was noted by real-time {PCR} in the fall and winter seasons, showing a significantly higher peak of rotavirus concentration at mean temperatures lower than {18\&\#xB0;C} and also higher, although not statistically different during drier weather. {VP7} and {VP4} gene characterization showed that G1 and P[8] genotypes were dominant, and temporal variations in genotype distribution were not observed. Rotavirus spread is complex and our results point out that weather factors alone cannot explain the seasonal quantitative pattern of the rotavirus disease. Therefore, alternative transmission routes, changes in human behavior and susceptibility, and the stability and survivability of the virus might all together contribute to the seasonality of rotavirus. The results obtained here provide evidence regarding the dynamics of rotavirus circulation and maintenance in Argentina.},
	doi = {10.1016/j.envres.2015.03.004},
	url = {http://dx.doi.org/10.1016/j.envres.2015.03.004},
	author = {Barril, PA and Fumian, TM and Prez, VE and Gil, PI and Mart{\'\i}nez, LC and Giordano, MO and Masachessi, G and Isa, MB and Ferreyra, LJ and R{\'e}, VE and Miagostovich, M and Pavan, JV and Nates, SV}
}
