

@article {8148,
	title = {{Dipstick test for rapid diagnosis of Shigella dysenteriae 1 in bacterial cultures and its potential use on stool samples}},
	journal = {PLoS One},
	volume = {6},
	number = {10},
	year = {2011},
	pages = {e24830},
	edition = {2011/10/11},
	abstract = {BACKGROUND: We describe a test for rapid detection of S. dysenteriae 1 in bacterial cultures and in stools, at the bedside of patients. METHODOLOGY/PRINCIPAL FINDINGS: The test is based on the detection of S. dysenteriae 1 lipopolysaccharide (LPS) using serotype 1-specific monoclonal antibodies coupled to gold particles and displayed on a one-step immunochromatographic dipstick. A concentration as low as 15 ng/ml of LPS was detected in distilled water and in reconstituted stools in 10 minutes. In distilled water and in reconstituted stools, an unequivocal positive reaction was obtained with 1.6x10(6) CFU/ml and 4.9x10(6) CFU/ml of S. dysenteriae 1, respectively. Optimal conditions to read the test have been determined to limit the risk of ambiguous results due to appearance of a faint yellow test band in some negative samples. The specificity was 100{\%} when tested with a battery of Shigella and unrelated strains in culture. When tested on 328 clinical samples in India, Vietnam, Senegal and France by laboratory technicians and in Democratic Republic of Congo by a field technician, the specificity (312/316) was 98.7{\%} (95{\%} CI:96.6-99.6{\%}) and the sensitivity (11/12) was 91.7{\%} (95{\%} CI:59.8-99.6{\%}). Stool cultures and the immunochromatographic test showed concordant results in 98.4 {\%} of cases (323/328) in comparative studies. Positive and negative predictive values were 73.3{\%} (95{\%} CI:44.8-91.1{\%}) and 99.7{\%} (95{\%} CI:98-100{\%}). CONCLUSION: The initial findings presented here for a simple dipstick-based test to diagnose S. dysenteriae 1 demonstrates its promising potential to become a powerful tool for case management and epidemiological surveys.},
	isbn = {1932-6203 (Electronic)
1932-6203 (Linking)},
	doi = {10.1371/journal.pone.0024830
PONE-D-11-05696 [pii]},
	author = {Taneja, N and Nato, F and Dartevelle, S and Sire, J M and Garin, B and L N Phuong and Diep, T T and Shako, J C and Bimet, F and Filliol, I and Muyembe, J J and Ungeheuer, M N and Ottone, C and Sansonetti, P and Germani, Y}
}
