

@article {8096,
	title = {{Production of a Shiga-like cytotoxin by Campylobacter.}},
	journal = {Microbial Pathogenesis},
	volume = {4},
	number = {6},
	year = {1988},
	month = {jun},
	pages = {455{\textendash}62},
	abstract = {<p>Cell lysates and culture supernatants of 36 Campylobacter isolates from patients with enteritis were tested for cytotoxic activity on HeLa cells. Cytotoxic activity was considered Shiga-like if neutralized by monoclonal antibody to the B subunit of Shiga-like toxin I of Escherichia coli and rabbit anti-Shiga toxin. Fifteen of the Campylobacter isolates produced no detectable cytotoxin, 10 produced a non-neutralizable cytotoxin, and 11 produced low levels of a cell-associated SLT. However, under low stringency conditions no hybridization was observed between a DNA fragment containing cloned SLT-I genes and restriction enzyme-digested total DNA from a Campylobacter strain that produced low levels of a Shiga-like toxin I. The Shiga-like toxin neutralizing titers in sera from 15 patients with C. jejuni infections, 5 patients infected with S. sonnei, and 20 healthy persons were then determined. No rise in neutralizing titer between acute and convalescent sera of patients with C. jejuni infection or S. sonnei infection was observed, although 27{\%} of C. jejuni-infected patients, 40{\%} of S. sonnei-infected patients, and 30{\%} of the healthy controls had neutralizing activity in their sera. These data indicate that low levels of Shiga-like toxin are produced by some Campylobacter isolates but that SLT is genetically distinct from the SLT-I toxin produced at high levels by certain E. coli. The findings also suggest that exposure to SLTs is common in the adult population but not as a consequence of infection with C. jejuni or S. sonnei.</p>
},
	keywords = {Adult, Antibodies, Bacterial, Bacterial Toxins, Bacterial Toxins: biosynthesis, Bacterial Toxins: immunology, Blotting, Campylobacter, Campylobacter: metabolism, Campylobacter: pathogenicity, Cytotoxins, Cytotoxins: biosynthesis, Cytotoxins: immunology, DNA, HeLa Cells, Humans, Monoclonal, Monoclonal: diagnostic use, Neutralization Tests, Nucleic Acid, Sequence Homology, Shiga Toxin 1, Southern, Species Specificity},
	issn = {0882-4010},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/3193876},
	author = {Moore, M A and Blaser, M J and Perez-Perez, G I and O{\textquoteright}Brien, A D}
}
