

@article {8054,
	title = {{Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease.}},
	journal = {Journal of Clinical Microbiology},
	volume = {41},
	number = {11},
	year = {2003},
	month = {nov},
	pages = {4930{\textendash}40},
	abstract = {<p>The distribution of EDL 933 O island 122 (OI-122) was investigated in 70 strains of Verocytotoxin-producing Escherichia coli (VTEC) of multiple serotypes that were classified into five \&quot;seropathotypes\&quot; (A through E) based on the reported occurrence of serotypes in human disease, in outbreaks, and/or in the hemolytic-uremic syndrome (HUS). Seropathotype A comprised 10 serotype O157:H7 and 3 serotype O157:NM strains. Seropathotype B (associated with outbreaks and HUS but less commonly than serotype O157:H7) comprised three strains each of serotypes O26:H11, O103:H2, O111:NM, O121:H19, and O145:NM. Seropathotype C comprised four strains each of serotypes O91:H21 and O113:H21 and eight strains of other serotypes that have been associated with sporadic HUS but not typically with outbreaks. Seropathotype D comprised 14 strains of serotypes that have been associated with diarrhea but not with outbreaks or HUS, and seropathotype E comprised animal VTEC strains of serotypes not implicated in human disease. All strains were tested for four EDL 933 OI-122 virulence genes (Z4321, Z4326, Z4332, and Z4333) by PCR. Negative PCRs were confirmed by Southern hybridization. Overall, 28 (40{\%}) strains contained OI-122 (positive for all four virulence genes), 27 (38.6{\%}) contained an \&quot;incomplete\&quot; OI-122 (positive for one to three genes), and 15 (21.4{\%}) strains did not contain OI-122. The seropathotype distribution of complete OI-122 was as follows: 100{\%} for seropathotype A, 60{\%} for B, 36{\%} for C, 15{\%} for D, and 0{\%} for E. The differences in the frequency of OI-122 between seropathotypes A, B, and C (associated with HUS) and seropathotypes D and E (not associated with HUS) and between seropathotypes A and B (associated with epidemic disease) and seropathotypes C, D, and E (not associated with epidemic disease) were highly significant (P {\textless} 0.0001).</p>
},
	keywords = {Animals, Bacterial, Blotting, Cattle, Cattle Diseases, Cattle Diseases: microbiology, Disease Outbreaks, Escherichia coli, Escherichia coli Infections, Escherichia coli Infections: epidemiology, Escherichia coli Infections: microbiology, Escherichia coli O157, Escherichia coli O157: genetics, Escherichia coli O157: pathogenicity, Escherichia coli: classification, Escherichia coli: genetics, Escherichia coli: pathogenicity, Genome, Humans, Incidence, Polymerase Chain Reaction, shiga toxins, Shiga Toxins: genetics, Southern, Virulence, Virulence: genetics},
	issn = {0095-1137},
	url = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=262514{\&}tool=pmcentrez{\&}rendertype=abstract},
	author = {Karmali, Mohamed A and Mascarenhas, Mariola and Shen, Songhai and Ziebell, Kim and Johnson, Shelley and Reid-Smith, Richard and Isaac-Renton, Judith and Clark, Clifford and Rahn, Kris and Kaper, James B}
}
