

@article {7975,
	title = {{Integrating conjugative elements of the SXT/R391 family trigger the excision and drive the mobilization of a new class of Vibrio genomic islands.}},
	journal = {Molecular microbiology},
	volume = {78},
	number = {3},
	year = {2010},
	month = {nov},
	pages = {576{\textendash}88},
	abstract = {In vibrios and enterobacteria lateral gene transfer is often facilitated by integrating conjugative elements (ICEs) of the SXT/R391 family. SXT/R391 ICEs integrate by site-specific recombination into prfC and transfer by conjugation, a process that is initiated at a specific locus called the origin of transfer (oriT(SXT) ). We identified genomic islands (GIs) harbouring a sequence that shares {\textgreater}63{\%} identity with oriT(SXT) in three species of Vibrio. Unlike SXT/R391 ICEs, these GIs are integrated into a gene coding for a putative stress-induced protein and do not appear to carry any gene coding for a conjugative machinery or for mobilization proteins. Our results show that SXT/R391 ICEs trigger the excision and mediate the conjugative transfer in trans of the three Vibrio GIs at high frequency. GIs{\textquoteright} excision is independent of the ICE-encoded recombinase and is controlled by the ICE-encoded transcriptional activator SetCD, which is expressed during the host SOS response. Both mobI and traI, two ICE-borne genes involved in oriT recognition, are essential for GIs{\textquoteright} transfer. We also found that SXT/R391 ICEs mobilize in trans over 1 Mb of chromosomal DNA located 5{\textquoteright} of the GIs{\textquoteright} integration site. Together these results support a novel mechanism of mobilization of GIs by ICEs of the SXT/R391 family.},
	issn = {1365-2958},
	doi = {10.1111/j.1365-2958.2010.07364.x},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/20807202},
	author = {Daccord, Aur{\'e}lie and Ceccarelli, Daniela and Burrus, Vincent}
}
