

@article {7779,
	title = {Specific Detection of Arcobacter and Campylobacter Strains in Water and Sewage by PCR and Fluorescent In Situ Hybridization},
	journal = {Applied and Environmental Microbiology},
	volume = {69},
	number = {2},
	year = {2003},
	note = {Specific Detection of Arcobacter and Campylobacter Strains in Water and Sewage by PCR and Fluorescent In Situ Hybridization; 6; NOT IN FILE; ID: 144},
	pages = {1181-1186},
	abstract = {The aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter and Campylobacter strains in river water and wastewater samples. Both 16S and 23S rRNA sequence data were used to design specific primers and oligonucleotide probes for PCR and FISH analyses, respectively. In order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on selective media. The detection range of PCR and FISH assays varied between 1 cell/ml (after enrichment) to 103 cells/ml (without enrichment). According to our results, both rRNA-based techniques have the potential to be used as quick and sensitive methods for detection of campylobacters in environmental samples},
	url = {http://aem.asm.org/cgi/content/abstract/69/2/1181 file://N:\YMB\TarjaP\Viitteet\Moreno.pdf},
	author = {Moreno,Yolanda and Botella,Salut and Alonso,Jose L. and Ferrus,Maria A. and Hernandez,Manuel and Hernandez,Javier}
}
