

@conference {722,
	title = {Biodegradation of Hydrocarbon Chains of Crude Oil Byproducts by Selected Protozoan Isolates in Polluted Wastewaters},
	year = {2014},
	publisher = {China University of Mining and Technology Press, Xuzhou,},
	organization = {China University of Mining and Technology Press, Xuzhou,},
	address = {China},
	abstract = {<div class="rtejustify">The demand of crude oil or any other oil by-products across the world and various activities are such as human and industrial activities has led to continuous pollution of various existing fresh water. The study was conducted using three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp) where their growth and biodegradation abilities were screened using crude oil spill rich polluted wastewater in shaking flasks at pH 8 and incubated at 30 {\textcelsius} with a speed of 100 rpm for 5 days. Chemical Oxygen Demand, Dissolved Oxygen and growth of protozoan isolates were determined using standard methods. Crude oil concentrations in the samples were analysed using partition gravimetric method. The study revealed that high amount of COD released and over 80\% DO were removed from wastewater. The protozoan biomass of 1.00{\texttimes}103 cells/mL was able to degrade crude oil (initial concentration:\&lt;50 mg/L) from wastewater at average rates ranging from 10.41\% to 55.5\%, from 11.0\% to 64.5\%, from 11.66\% to 44.21\% and from 21.2\% to 61.1\% for Aspidisca sp.,Trachelophyllum sp., Peranema sp., and a consortium of the three isolates, respectively.An increase in protozoan biomasses of 1.00{\texttimes}105 cells/mL and 1.00{\texttimes}106 cells/mL resulted in oil biodegradation rates ranging from 15.4\% to 71.0\%, 15.0\% to 68.9\%, 13.0\% to 56.0\% and 28.0\% to 85.2\%, respectively. However, an increase in oil concentrations in wastewater mixed liquor (\&gt;50 mg/L) decreased their biodegradation capacity to \&gt;60\%. Statistically, the microbes were found to have a significant difference in their biodegradation ability in different various oily wastewater concentrations (p\&lt;0.001).</div>
},
	keywords = {biodegradation, crude (petroleum) oil, protozoan biomass, wastewater},
	isbn = {978-7-5646-2437-8},
	url = {https://www.imwa.info/docs/imwa_2014/IMWA2014_Kachienga_743.pdf},
	author = {M NB Momba and L. Kachienga}
}
