

@inbook {544,
	title = {Detection of four adenovirus serotypes within water-isolated strains of Acanthamoeba in the Canary Islands, Spain},
	booktitle = {Am J Trop Med Hyg},
	volume = {77},
	year = {2007},
	note = {Lorenzo-Morales, Jacob Coronado-Alvarez, Nieves Martinez-Carretero, Enrique Maciver, Sutherland K Valladares, Basilio Research Support, Non-U.S. Gov{\textquoteright}t The American journal of tropical medicine and hygiene 77/4/753 [pii] Am J Trop Med Hyg. 2007 Oct;77(4):753-6.},
	pages = {753-6},
	type = {Book Section},
	address = {United States},
	abstract = {We surveyed 236 potentially pathogenic Acanthamoeba strains, isolated from water sources in the Canary Islands, for the presence of human adenoviruses (HAdV) using a polymerase chain reaction (PCR)-based typing assay. A total of 34 of these strains were found to be positive for adenovirus belonging to four different HAdV serotypes (HAdV-1, 2, 8, and 37). We found that HAdV-2 was the most frequently encountered serotype amongst the Acanthamoeba strains, and their identification was confirmed by a nested PCR specific for this serotype. We showed that Acanthamoeba genotype T4 was highly associated with serotype HAdV-2, whereas Acanthamoeba genotype T3 was most often associated with adenovirus serotypes related to ocular diseases. Based on these data, we suggest that Acanthamoeba should be considered as a potential reservoir and perhaps even a transmitter of adenoviruses to human and other secondary hosts.},
	keywords = {Acanthamoeba/ isolation \& purification/ virology Adenoviridae/classification/ isolation \& purification/pathogenicity Amebiasis/genetics/immunology Animals Humans Polymerase Chain Reaction/methods Spain Water Microbiology},
	isbn = {0002-9637 (Print) 0002-9637 (Linking)},
	author = {Lorenzo-Morales, J. and Coronado-Alvarez, N. and Martinez-Carretero, E. and Maciver, S. K. and Valladares, B.}
}
