

@article {539,
	title = {Characterizations of adenovirus type 41 isolates from children with acute gastroenteritis in Japan, Vietnam, and Korea},
	journal = {J Clin Microbiol},
	volume = {42},
	number = {9},
	year = {2004},
	note = {Li, Lei Shimizu, Hideaki Doan, Lan Thi Phuong Tung, Phan Gia Okitsu, Shoko Nishio, Osamu Suzuki, Eiko Seo, Jeong Kee Kim, Kyo Sun Muller, Werner E G Ushijima, Hiroshi Research Support, Non-U.S. Gov{\textquoteright}t United States Journal of clinical microbiology},
	pages = {4032-9},
	type = {Journal Article},
	abstract = {Genetic and antigenic characterizations of 70 strains of adenovirus type 41 (Ad41), isolated between 1998 and 2001 from children in Japan, Vietnam, and Korea, were done by DNA restriction enzyme (RE) analysis, sequencing analysis, and monoclonal antibody (MAb)-based enzyme-linked immunosorbent assay (ELISA). Eight genome types were observed in the present study, among which D25, D26, D27, and D28 were novel genome types. These eight genome types were divided into two genome-type clusters (GTCs) based on phylogenetic analysis of the hypervariable regions (HVRs) of the hexon. GTC1 includes D1, D25, D26, D27, and D28, and the GTC2 contains D4, D12, and D22. The amino acid homologies among the members within a GTC were 97 to 100\%, whereas between the members of different GTCs the homologies were 92 to 94\%. The specificity of the GTC classification was confirmed by ELISA with MAb 1F, which was selected by the Ad41 prototype Tak strain. It was found that only the isolates of GTC1 but not of GTC2 reacted with MAb 1F. These results suggest that Ad41 isolates from the three countries should be classified into two subtypes. The accumulation of amino acid mutations located in HVRs of hexon are indicative for the classification of Ad41 subtype.},
	keywords = {Acute Disease Adenoviruses, Amino Acid Vietnam, Human/classification/genetics/ isolation \& purification Amino Acid Sequence Base Sequence Child DNA Primers Enzyme-Linked Immunosorbent Assay/methods Gastroenteritis/ virology Genome, Viral Humans Japan Korea Molecular Sequence Data Phylogeny Polymerase Chain Reaction/methods Restriction Mapping Sequence Alignment Sequence Homology},
	issn = {0095-1137 (Print) 0095-1137 (Linking)},
	author = {Li, L. and Shimizu, H. and Doan, L. T. and Tung, P. G. and Okitsu, S. and Nishio, O. and Suzuki, E. and Seo, J. K. and Kim, K. S. and Muller, W. E. and Ushijima, H.}
}
