

@article {516,
	title = {Safe adoptive transfer of virus-specific T-cell immunity for the treatment of systemic adenovirus infection after allogeneic stem cell transplantation},
	journal = {Br J Haematol},
	volume = {134},
	number = {1},
	year = {2006},
	note = {Feuchtinger, Tobias Matthes-Martin, Susanne Richard, Celine Lion, Thomas Fuhrer, Monika Hamprecht, Klaus Handgretinger, Rupert Peters, Christina Schuster, Friedhelm R Beck, Robert Schumm, Michael Lotfi, Ramin Jahn, Gerhard Lang, Peter Journal Article Research Support, Non-U.S. Gov{\textquoteright}t England Br J Haematol. 2006 Jul;134(1):64-76.},
	pages = {64-76},
	type = {Journal Article},
	abstract = {During periods of immunosuppression, such as postallogeneic stem cell transplantation (SCT), patients are at significant risk for severe viral infections. Human adenovirus (HAdV) infection is a serious complication post-SCT, especially in children. Virus-specific T cells are essential for the clearance of HAdV, as antiviral chemotherapy has revealed limited success. We present feasibility data for a new treatment option using virus-specific donor T cells for adoptive transfer of immunity to patients with HAdV-infection/reactivation. Virus-specific donor T cells were isolated and infused into nine children with systemic HAdV infection after SCT. Isolation was based on gamma-interferon (IFN-gamma) secretion after short in vitro stimulation with viral antigen, resulting in a combination of CD4(+) and CD8(+) T cells. 1.2-50 x 10(3)/kg T cells were infused for adoptive transfer. Isolated cells showed high specificity and markedly reduced alloreactivity in vitro. Adoptive transfer of HAdV-specific immunity was successful in five of six evaluable patients, documented by a dose-independent and sustained in vivo expansion of HAdV-specific T cells, associated with a durable clearance/decrease of viral copies. T-cell infusion was well tolerated in all nine patients, except one case with graft-versus-host disease II of the skin. In conclusion, induction of a specific T-cell response through adoptive transfer was feasible and effective. When performed early in the course of infection, adoptive T-cell transfer may protect from HAdV-related complications.},
	keywords = {Adenovirus Infections, Homologous Treatment Outcome, Human/*immunology Adoptive Transfer/*methods CD4-Positive T-Lymphocytes/immunology/virology CD8-Positive T-Lymphocytes/immunology/virology Cell Proliferation Cell Separation/methods Child Child, Human/immunology/*therapy Adenoviruses, Preschool Feasibility Studies Hematopoietic Stem Cell Transplantation/*adverse effects Humans Infant T-Lymphocytes/immunology/*virology Transplantation},
	issn = {0007-1048 (Print) 0007-1048},
	doi = {10.1111/j.1365-2141.2006.06108.x},
	author = {Feuchtinger, T. and Matthes-Martin, S. and Richard, C. and Lion, T. and Fuhrer, M. and Hamprecht, K. and Handgretinger, R. and Peters, C. and Schuster, F. R. and Beck, R. and Schumm, M. and Lotfi, R. and Jahn, G. and Lang, P.}
}
