

@inbook {509,
	title = {A novel conditionally replicating adenoviral vector with dual expression of IL-24 and arresten inserted in E1 and the region between E4 and fiber for improved melanoma therapy},
	booktitle = {Cancer Gene Ther},
	volume = {19},
	year = {2012},
	note = {Chai, L Liu, S Mao, Q Wang, D Li, X Zheng, X Xia, H Research Support, Non-U.S. Gov{\textquoteright}t Cancer gene therapy cgt201184 [pii] Cancer Gene Ther. 2012 Apr;19(4):247-54. doi: 10.1038/cgt.2011.84. Epub 2011 Dec 23.},
	pages = {247-54},
	type = {Book Section},
	address = {England},
	abstract = {Although the combination of gene therapy and virotherapy for cancer therapy has obtained some encouraging results in vitro and in vivo over the past few years, some improvements of the vectors are still urgently needed to enhance their therapeutic effects for cancers. In order to maximize the anti-cancer activities of conditionally replicating adenoviral vectors (CRAd) vector, we for the first time generated a novel CRAd vector by inserting an expression cassette between E4 and the fiber using homologous recombination and tested this vector in melanoma cancer therapy. By using this novel vector we expressed two therapeutic transgenes, IL-24 and arresten, inserted in E1 and the region between E4 and the fiber, respectively, to test the melanoma inhibitory activities of this oncolytic virus in vitro and in vivo. The two therapeutic transgenes were successfully expressed by the novel CRAd, which was confirmed by western blotting. We then showed that this novel CRAd vector significantly suppressed the tumor growth of melanoma in vitro and in vivo compared with the control by inducing apoptosis and inhibiting angiogenesis. The novel CRAd vector generated in this study holds promise for developing more effective therapeutics for not only melanoma but also other common cancers.},
	keywords = {Adenoviridae/ genetics Animals Cell Line Cell Proliferation Collagen Type IV/ genetics Female Gene Expression Gene Order Genetic Therapy Genetic Vectors/ genetics Humans Interleukins/ genetics/metabolism Melanoma, Experimental/ genetics/ therapy Mice Mice, Nude Oncogene Proteins, Viral Viral Proteins/genetics Xenograft Model Antitumor Assays},
	isbn = {1476-5500 (Electronic) 0929-1903 (Linking)},
	doi = {10.1038/cgt.2011.84},
	author = {Chai, L. and Liu, S. and Mao, Q. and Wang, D. and Li, X. and Zheng, X. and Xia, H.}
}
