

@article {4250,
	title = {{Distinct behaviors of infectious F-specific RNA coliphage genogroups at a wastewater treatment plant.}},
	journal = {The Science of the Total Environment},
	volume = {520},
	year = {2015},
	month = {jul},
	pages = {32{\textendash}8},
	abstract = {<p>The present study aimed to determine the differences in the behaviors of four F-specific RNA (F-RNA) coliphage genogroups (GI-GIV) during wastewater treatment. Raw sewage, aeration tank effluent, secondary-treated sewage, and return activated sludge were collected from a wastewater treatment plant in Japan at monthly intervals between March and December 2011 (n=10 each). F-specific coliphages were detected by plaque assay in all tested samples, with a concentration ranging from -0.10 to 3.66 log10 plaque-forming units/ml. Subsequently, eight plaques were isolated from each sample, followed by genogroup-specific reverse-transcription quantitative PCR (qPCR) for F-RNA coliphages and qPCR for F-specific DNA (F-DNA) coliphages. GI F-RNA coliphages were the most abundant in the secondary-treated sewage samples (73{\%} of the plaque isolates), while GII F-RNA coliphages were the most abundant in the other three sample types (41-81{\%}, depending on sample type). Based on the results of the quantification and genotyping, the annual mean concentrations of each F-specific coliphage type were calculated, and their reduction ratios during wastewater treatment were compared with those of indicator bacteria (total coliforms and Escherichia coli) and enteric viruses (human adenoviruses and GI and GII noroviruses). The mean reduction ratio of GI F-RNA coliphages was the lowest (0.93 log10), followed by those of the indicator bacteria and enteric viruses (1.59-2.43 log10), GII-GIV F-RNA coliphages ({\textgreater}2.60-3.21 log10), and F-DNA coliphages ({\textgreater}3.41 log10). These results suggest that GI F-RNA coliphages may be used as an appropriate indicator of virus reduction during wastewater treatment.</p>
},
	keywords = {Coliphages, Coliphages: classification, Coliphages: genetics, Fluid, genotype, Japan, RNA, Waste Disposal, Waste Water, Waste Water: virology, Water Microbiology},
	issn = {1879-1026},
	doi = {10.1016/j.scitotenv.2015.03.034},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/25791054},
	author = {Haramoto, Eiji and Fujino, Saki and Otagiri, Mikie}
}
