

@article {3864,
	title = {Detection of bovine group B rotaviruses in feces by polymerase chain reaction.},
	journal = {Journal of Veterinary Diagnostic Investigation},
	volume = {6},
	number = {3},
	year = {1994},
	pages = {302{\textendash}307},
	abstract = {<p>A pair of primers designed from the sequence of genome segment 9 of group B rat rotavirus \{\{\}(IDIR)\{\}\} were employed to amplify genome segment 9 of a group B bovine rotavirus in a polymerase chain reaction \{\{\}(PCR)\{\}\} and to sequence the derived \{\{\}PCR\{\}\} products. A new pair of primers were synthesized from the obtained sequence data and used in a \{\{\}PCR\{\}\} detection assay for group B bovine rotavirus in fecal samples. In addition, another pair of primers were designed to produce a \{\{\}PCR-derived\{\}\} internal probe. This probe was used in a chemiluminescent hybridization to confirm the specificity and to increase the sensitivity of the assay. This assay could detect 0.1 fg of target double-stranded \{\{\}RNA.\{\}\} It was specific to group B bovine rotavirus and did not detect group B rat \{\{\}(IDIR)\{\}\} and porcine rotaviruses, group A bovine \{\{\}(NCDV),\{\}\} simian \{\{\}(SA-11),\{\}\} equine \{\{\}(H-2),\{\}\} porcine \{\{\}(OSU),\{\}\} human \{\{\}(DS-1),\{\}\} deer, and avian rotaviruses, coronavirus, or other enteric organisms tested in this study.</p>
},
	author = {Chinsangaram, J and Akita, G Y and Osburn, B I}
}
