

@article {3772,
	title = {Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment.},
	journal = {Food and Environmental Virology},
	year = {2015},
	abstract = {<p>The objective of this study is to compare the prevalence of rotaviruses groups A and C in Egyptian children and aquatic environment. From 110 stool specimens of children with acute diarrhea and using \{\{\}RT-PCR,\{\}\} 35 samples \{\{\}(31.8\{\&amp;\}\{\#\}xA0;\{\%\})\{\}\} were positive for human rotavirus group A and 15 samples \{\{\}(13.6\{\&amp;\}\{\#\}xA0;\{\%\})\{\}\} were positive for human rotavirus group C. From 96 samples collected from Zenin wastewater treatment plant over a 2-year period \{\{\}(November\{\}\} \{\{\}2009-October\{\}\} 2011) and using \{\{\}RT-PCR,\{\}\} rotavirus group A was detected in (4/24) \{\{\}16.7\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} (5/24) \{\{\}20.8\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} (4/24) \{\{\}16.7\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} and (4/24) \{\{\}16.7\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} while rotavirus group C was detected in (2/24) \{\{\}8.3\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} (3/24) \{\{\}12.5\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} (3/24) \{\{\}12.5\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} and (0/24) \{\{\}0\{\&amp;\}\{\#\}xA0;\{\%\}\{\}\} in raw sewage, after primary sedimentation, after secondary sedimentation, and after final chlorination, respectively. Moreover, from 96 samples collected from \{\{\}El-Giza\{\}\} water treatment plant over a 2-year period \{\{\}(November\{\}\} \{\{\}2009-October\{\}\} 2011), rotavirus group A was detected in (7/24) \{\{\}29.2\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} (6/24) \{\{\}25\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} (5/24) \{\{\}20.8\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} and (3/24) \{\{\}12.5\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} while rotavirus group C was detected in (3/24) \{\{\}12.5\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} (1/24) \{\{\}4.2\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} (1/24) \{\{\}4.2\{\&amp;\}\{\#\}xA0;\{\%\},\{\}\} and (0/24) \{\{\}0\{\&amp;\}\{\#\}xA0;\{\%\}\{\}\} in raw Nile water, after sedimentation, after sand filtration, and after final chlorination, respectively. Using \{\{\}SYBR\{\}\} Green real-time \{\{\}RT-PCR,\{\}\} the number of human rotavirus group A genome or infectious units was higher than rotavirus group C. \{\{\}VP6\{\}\} sequence analysis of the \{\{\}RT-PCR\{\}\} positive rotavirus group C samples revealed that four clinical specimens and three environmental samples showed similar sequences clustered with \{\{\}Moduganari/Human\{\}\} Nigerian strain \{\{\}AF\{\}\} 325806 with \{\{\}98\{\&amp;\}\{\#\}xA0;\{\%\}\{\}\} homology, and two clinical specimens and one environmental sample showed similar sequences clustered with Dhaka \{\{\}CB/Human\{\}\} Bangladesh strain \{\{\}AY\{\}\} 754826 with \{\{\}97\{\&amp;\}\{\#\}xA0;\{\%\}\{\}\} homology.</p>
},
	doi = {10.1007/s12560-015-9184-6},
	url = {http://dx.doi.org/10.1007/s12560-015-9184-6},
	author = {M El-Senousy, Waled and Ragab, Ahmed M and Handak, Eman M}
}
