

@article {3648,
	title = {Rapid detection of infectious adenoviruses by \{\{\}{mRNA}\{\}\} real-time \{\{\}{RT}-{PCR}.\{\}\}},
	journal = {Journal of virological methods},
	volume = {127},
	number = {2},
	year = {2005},
	pages = {148{\textendash}153},
	abstract = {Adenoviruses are among the most persistent and ubiquitous viruses in water and wastewater, but some of them are difficult to detect due to non-cytopathogenicity and slow growth in cell cultures. A \{\{\}TaqMan\{\}\} real-time \{\{\}RT-PCR\{\}\} method in conjunction with cell culture infectivity was developed to rapidly detect \{\{\}mRNA\{\}\} produced by infectious adenoviruses in water samples. Only infectious adenoviruses were detected by this method, based on their ability to produce \{\{\}mRNA\{\}\} during replication in cell culture. The \{\{\}mRNA\{\}\} of Ad41 was detected as soon as 3 days after infection at levels as low as 5 infectious units \{\{\}(IU)\{\}\} per cell culture. In order to confirm that our methods detected only infectious viruses, 1-ml volumes of \{\{\}10(4)IU\{\}\} of Ad41 were exposed to different free chlorine doses. No \{\{\}mRNA\{\}\} was detected in cells inoculated with Ad41 treated with the highest free chlorine dose of 100 mg min/l. However, \{\{\}mRNA\{\}\} of adenovirus was detected in cells inoculated with virus that was untreated or exposed to a lower free chlorine dose of 1 mg min/l. These results suggest that \{\{\}mRNA\{\}\} detection by real-time \{\{\}RT-PCR\{\}\} is a sensitive and specific method to detect low levels of infectious adenoviruses in water and other environmental media within 1-3 days.},
	doi = {10.1016/j.jviromet.2005.02.017},
	url = {http://dx.doi.org/10.1016/j.jviromet.2005.02.017},
	author = {G Ko and Jothikumar, Narayanan and Hill, Vincent R and Sobsey, Mark D}
}
