

@article {3268,
	title = {{Genetic diversity of noroviruses in raw and treated sewage water}},
	journal = {Research in Microbiology},
	volume = {156},
	number = {4},
	year = {2005},
	pages = {532{\textendash}540},
	abstract = {Human noroviruses cause gastroenteritis in humans, leading to high virus loads in sewage. Norovirus concentrations in raw and treated sewage samples from two sewage treatment plants (STP) were studied, along with virus removal and genetic diversity. Over one year, the average norovirus concentrations in raw sewage were approximately 105 pcr detectable units (pdu) per liter compared with 103 pdu/l of treated sewage. Similar sewage treatment processes at STP-A and STP-B led to 2.7 and 2.0log10-units of virus removal, respectively. In total, 11 different norovirus variants were detected in 49 out of 53 sewage samples, with up to four different norovirus strains in a single sewage sample. Along with GGI.6 Sindlesham and GGII.2 Melksham, the GGIIb variant was one of the most prevalent noroviruses in both raw and treated sewage. This strain emerged among populations in Europe in 2000 and 2001. Treated sewage containing 10 2-103 norovirus pdu is discharged into the surface water. The use of such fecally contaminated surface waters for shellfish culture, drinking water production and recreational purposes poses a potential health risk. We showed the presence of multiple norovirus strains in raw and treated sewage, confirming the need to clone before sequencing the RT-PCR products. Exposure to multiple norovirus strains in sewage contaminated food or water may lead to the occurrence of norovirus recombinants, which may be more virulent and pathogenic than the norovirus strains already circulating in the population. ?? 2005 Elsevier SAS. All rights reserved.},
	keywords = {Calicivirus, Norovirus, Sequence, Sewage, Treatment},
	isbn = {0923-2508},
	issn = {09232508},
	doi = {10.1016/j.resmic.2005.01.008},
	author = {Van Den Berg, Harold and Lodder, Willemijn and Van Der Poel, Wim and Vennema, Harry and Ana M. De Roda Husman}
}
