

@article {2657,
	title = {VP8*P[8] lineages of group A rotaviruses circulating over 20 years in Brazil: proposal of six different sub-lineages for P[8]-3 clade.},
	journal = {Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases},
	volume = {16},
	year = {2013},
	pages = {200-205},
	abstract = {<p>Group A rotaviruses {(RVA)} is the most important cause of severe gastroenteritis among children worldwide. Vaccination is considered the best alternative among public health measures to reduce and prevent the global burden caused by {RVA} infections. Rotarix\&amp;\#x2122;, a monovalent vaccine based on a human strain with a {G1P[8]-1} specificity, was introduced in the National Brazilian Immunization Programs {(NIP)} in March, 2006. {RVA} P[8] is the most prevalent P genotype worldwide and four distinct phylogenetic lineages: P[8]-1, -2, -3, and -4 have been described. In the current study phylogenetic analysis of the {VP8(*)} gene of 135 {RVA} P[8] Brazilian strains, in combination with G1, G3, G5 or G9 {VP7} genotype, collected from 1986 to 2011 were carried out for a better understanding of the evolution of this viral genotype in Brazil. Lineages P[8]-1, P[8]-2, and P[8]-3 were observed circulating in Brazil. In 2001 these three P[8] lineages co-circulated simultaneously and this is the first report in South America to date. Considering the P[8] lineage and the G genotype, all G3 strains were related to lineage P[8]-3, whereas the G9 strains were related to P[8]-2 and P[8]-3 and G1 and G5 were related to P[8]-1, P[8]-2, and P[8]-3. In addition, the phylogenetic analysis based on estimate of genetic distances between P[8]-3 strains and the definition of a 1.5\% cutoff value (with relevant statistical support) it was possible to propose a new classification for the P[8]-3 lineage into six different sub-lineages: P[8]-3.1 to P[8]-3.6. These findings reinforce the notion of the existence of constraints within specific {RVA} strains populations. The results obtained in this study reinforce the importance of a continuous {RVA} surveillance of circulating strains in order to predict the possible variants that will circulate in a country, assess the effects of vaccination on {RVA} circulating strains, and ultimately help in the design, challenges, and prospects of {RVA} vaccines.</p>
},
	doi = {10.1016/j.meegid.2013.01.004},
	url = {http://dx.doi.org/10.1016/j.meegid.2013.01.004},
	author = {da Silva, Marcelle F and G{\'o}mez, Mariela M and Rose, Tatiana L and Eduardo M Volot{\~a}o and Carvalho-Costa, Filipe A and Bello, Gonzalo and Leite, Jos{\'e} P}
}
