

@article {2559,
	journal = {Current protocols in microbiology},
	volume = {Chapter 15},
	year = {2009},
	pages = {Unit 15C.3},
	abstract = {Group A rotaviruses {(RVs)} infect the young of numerous animal species and cause acute gastroenteritis. Cultivation of animal and human {RVs} in cells requires proteolytic activation of the viral attachment protein using trypsin. Continuous cell lines, such as rhesus monkey kidney cells, as well as primary monkey kidney cells, are routinely used for the growth and characterization of {RVs.} Isolation and cultivation of human {RVs} from clinical fecal specimens is difficult and adaptation to growth in vitro requires multiple rounds of passage in primary cells. Following growth, {RV} stocks can be purified by centrifugation, if required, and quantified using plaque assay or fluorescence focus assay. This unit describes easily applicable procedures for the culturing, storage, and quantification of {RVs.}},
	doi = {10.1002/9780471729259.mc15c03s15},
	url = {http://dx.doi.org/10.1002/9780471729259.mc15c03s15},
	author = {Arnold, Michelle and Patton, John T and McDonald, Sarah M}
}
