

@article {2515,
	title = {G1P[8] species A rotavirus over 27 years{\textendash}pre- and post-vaccination eras{\textendash}in Brazil: full genomic constellation analysis and no evidence for selection pressure by Rotarix{\textregistered} vaccine.},
	journal = {Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases},
	volume = {30},
	year = {2015},
	pages = {206-218},
	abstract = {<p>Epidemiological data on species A rotavirus {(RVA)} infections have demonstrated the genetic diversity of strains circulating worldwide. Many G and P genotype combinations have been described over the years, varying regionally and temporally, especially in developing countries. However, the most common G and P genotype combinations identified in {RVA} human strains worldwide are {G1P[8],} {G2P[4],} {G3P[8],} {G4P[8]} and {G9P[8].} {RVA} genotype {G1P[8]} strains are responsible for more than 50\% of child infections worldwide and component of the two vaccines {(Rotarix\&amp;\#xAE;} {[RV1]} and {RotaTeq\&amp;\#xAE;} {[RV5])} licensed globally. For a better understanding of the evolutionary mechanisms of this genotype in Brazil, phylogenetic analyses based on the 11 {RVA} genome segments (genomic constellation) from 90 {G1P[8]} {RVA} strains collected in two eras - (i) pre-vaccination with {RV1} {(1996-February} 2006); (ii) post-vaccination {(March} 2006-2013) - in different Brazilian states were performed. The results showed the Wa-like genomic constellation of the Brazilian {G1P[8]} strains with a {I1-R1-C1-M1-A1-N1-T1-E1-H1} specificity, except for two strains (rj14055-07 and ba19030-10) that belong to a {I1-R1-C1-M1-A1-N1-T3-E1-H1} genomic constellation, evidencing the occurrence of reassortment {(Wa-like\&amp;\#xD7;AU-1-like)} of the {NSP3} gene. Reassortment events were also demonstrated between Brazilian {G1P[8]} strains and the {RV1} vaccine strain in some genes in vaccinated and unvaccinated children. {VP7} and {VP8*} antigenic site analysis showed that the amino acid substitutions observed in samples collected after the introduction of {RV1} in Brazil were already detected in samples collected in the 1980s and 1990s, suggesting that mass Brazilian {RV1} vaccination had no impact on the diversity observed inside antigenic sites for these two proteins.</p>
},
	doi = {10.1016/j.meegid.2014.12.030},
	url = {http://dx.doi.org/10.1016/j.meegid.2014.12.030},
	author = {Silva, Marcelle F and Rose, Tatiana L and G{\'o}mez, Mariela M and Carvalho-Costa, Filipe A and Fialho, Alexandre M and Assis, Rosane M and Andrade, Juliana da S and Eduardo M Volot{\~a}o and Leite, Jos{\'e} P P}
}
