

@article {2157,
	title = {{Production of Shiga toxin and other cytotoxins by serogroups of Shigella.}},
	journal = {The Journal of infectious diseases},
	volume = {154},
	number = {6},
	year = {1986},
	pages = {996{\textendash}1002},
	abstract = {We measured the cytotoxic activity of 119 strains of Shigella by using a quantitative [3H]thymidine-labeled HeLa cell assay. We assayed 13 strains of Shigella dysenteriae 1; 18 strains of S. dysenteriae types 2 and 3; and 88 strains of Shigella sonnei, Shigella flexneri, and Shigella boydii. Strains of S. dysenteriae 1 demonstrated high levels of cytotoxicity (geometric mean, 10(5.04) CD50/mg of protein; range, 10(3.95)-10(6.10). Cytotoxic activities of the non-type 1 strains of S. dysenteriae and of the other Shigella serogroups were approximately 1/1,000 that of the S. dysenteriae 1 strains (range, 10(1.09)-10(3.11) CD50/mg of protein). Neutralization of cytotoxicity by using rabbit antiserum to purified Shiga toxin revealed that in all strains of S. dysenteriae 1, greater than or equal to 99.5\% of cytotoxic activity was attributable to Shiga toxin. In contrast, 88 of the other Shigella strains produced only nonneutralizable cytotoxic activity. Six of 18 strains of non-type 1 S. dysenteriae and 12 of 88 strains from other Shigella serogroups produced both Shiga toxin and nonneutralizable toxin.},
	keywords = {Bacterial Toxins, Bacterial Toxins: biosynthesis, Bacterial Toxins: immunology, Cytotoxins, Cytotoxins: biosynthesis, Escherichia coli, Escherichia coli: pathogenicity, HeLa Cells, shiga toxins, Shigella, Shigella: classification, Shigella: pathogenicity, Species Specificity},
	issn = {0022-1899},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/3537154},
	author = {Bartlett, A V and Prado, D and Cleary, T G and Pickering, L K}
}
