

@article {1949,
	title = {RNA viral community in human feces: prevalence of plant pathogenic viruses},
	journal = {PLoS Biol},
	volume = {4},
	number = {1},
	year = {2006},
	note = {Zhang, Tao Breitbart, Mya Lee, Wah Heng Run, Jin-Quan Wei, Chia Lin Soh, Shirlena Wee Ling Hibberd, Martin L Liu, Edison T Rohwer, Forest Ruan, Yijun eng Research Support, Non-U.S. Gov{\textquoteright}t Research Support, U.S. Gov{\textquoteright}t, Non-P.H.S. 2005/12/13 09:00 PLoS Biol. 2006 Jan;4(1):e3.},
	pages = {e3},
	type = {Journal Article},
	abstract = {The human gut is known to be a reservoir of a wide variety of microbes, including viruses. Many RNA viruses are known to be associated with gastroenteritis; however, the enteric RNA viral community present in healthy humans has not been described. Here, we present a comparative metagenomic analysis of the RNA viruses found in three fecal samples from two healthy human individuals. For this study, uncultured viruses were concentrated by tangential flow filtration, and viral RNA was extracted and cloned into shotgun viral cDNA libraries for sequencing analysis. The vast majority of the 36,769 viral sequences obtained were similar to plant pathogenic RNA viruses. The most abundant fecal virus in this study was pepper mild mottle virus (PMMV), which was found in high concentrations{\textendash}up to 10(9) virions per gram of dry weight fecal matter. PMMV was also detected in 12 (66.7\%) of 18 fecal samples collected from healthy individuals on two continents, indicating that this plant virus is prevalent in the human population. A number of pepper-based foods tested positive for PMMV, suggesting dietary origins for this virus. Intriguingly, the fecal PMMV was infectious to host plants, suggesting that humans might act as a vehicle for the dissemination of certain plant viruses.},
	keywords = {Adult Capsicum/virology Feces/*virology Food/virology Humans Infant Molecular Sequence Data Plant Viruses/*isolation \& purification RNA Viruses/*isolation \& purification Reverse Transcriptase Polymerase Chain Reaction},
	issn = {1545-7885 (Electronic) 1544-9173 (Linking)},
	doi = {10.1371/journal.pbio.0040003},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/16336043},
	author = {Zhang, T. and Breitbart, M. and Lee, W. H. and Run, J. Q. and Wei, C. L. and Soh, S. W. and Hibberd, M. L. and Liu, E. T. and Rohwer, F. and Ruan, Y.}
}
