

@article {1822,
	title = {Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR},
	journal = {Appl Environ Microbiol},
	volume = {61},
	number = {2},
	year = {1995},
	note = {Schwab, K J De Leon, R Sobsey, M D eng Comparative Study Research Support, Non-U.S. Gov{\textquoteright}t Research Support, U.S. Gov{\textquoteright}t, Non-P.H.S. 1995/02/01 Appl Environ Microbiol. 1995 Feb;61(2):531-7.},
	pages = {531-7},
	type = {Journal Article},
	abstract = {In this study we developed a concentration and purification procedure to facilitate reverse transcription (RT)-PCR detection of enteric viruses in water sample concentrates obtained by conventional filter adsorption-elution methods. One liter of beef extract-glycine eluate with or without humic acid and seeded with poliovirus type 1, hepatitis A virus, and Norwalk virus was used as a model system, and the eluent was further processed for RT-PCR compatibility. The sample concentration and purification procedures which we used included polyethylene glycol precipitation, Pro-Cipitate precipitation, a second polyethylene glycol precipitation, spin column chromatography, and ultrafiltration. The sample volumes were reduced from 1 liter to 20 to 50 microliters, and the samples were purified enough so that viruses could be detected by the RT-PCR. The ability to detect low levels of enteric viruses by molecular techniques was compared directly with the ability to detect enteric viruses by cell culture infectivity procedures. As little as 3 PFU of poliovirus type 1 in an initial 1 liter of mock eluate was detected by the RT-PCR.},
	keywords = {Animals Base Sequence Cattle Chemical Precipitation DNA Primers/genetics DNA, Viral/genetics Enterovirus/genetics/*isolation \& purification Evaluation Studies as Topic Hepatovirus/genetics/*isolation \& purification Humans Molecular Sequence Data Norwalk virus/genetics/*isolation \& purification Oligonucleotide Probes Polyethylene G},
	issn = {0099-2240 (Print) 0099-2240 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/7574592},
	author = {Schwab, K. J. and De Leon, R. and Sobsey, M. D.}
}
