

@article {1795,
	title = {Bacterial lipopolysaccharide binding enhances virion stability and promotes environmental fitness of an enteric virus},
	journal = {Cell Host and Microbe},
	volume = {15},
	number = {1},
	year = {2014},
	note = {Robinson, Christopher M Jesudhasan, Palmy R Pfeiffer, Julie K eng R01 AI074668/AI/NIAID NIH HHS/ R01 AI74668/AI/NIAID NIH HHS/ T32 AI007520/AI/NIAID NIH HHS/ T32 AI070116/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov{\textquoteright}t 2014/01/21 06:00 Cell Host Microbe. 2014 Jan 15;15(1):36-46. doi: 10.1016/j.chom.2013.12.004.},
	pages = {36-46},
	type = {Journal Article},
	abstract = {<p>Enteric viruses, including poliovirus and reovirus, encounter a vast microbial community in the mammalian gastrointestinal tract, which has been shown to promote virus replication and pathogenesis. Investigating the underlying mechanisms, we find that poliovirus binds bacterial surface polysaccharides, which enhances virion stability and cell attachment by increasing binding to the viral receptor. Additionally, we identified a poliovirus mutant, VP1-T99K, with reduced lipopolysaccharide (LPS) binding. Although T99K and WT poliovirus cell attachment, replication, and pathogenesis in mice are equivalent, VP1-T99K poliovirus was unstable in feces following peroral inoculation of mice. Consequently, the ratio of mutant virus in feces is reduced following additional cycles of infection in mice. Thus, the mutant virus incurs a fitness cost when environmental stability is a factor. These data suggest that poliovirus binds bacterial surface polysaccharides, enhancing cell attachment and environmental stability, potentially promoting transmission to a new host.</p>
},
	keywords = {Animals Cell Line, Transgenic Microbial Interactions/*genetics Mutation Poliomyelitis/metabolism/mortality/*virology Poliovirus/genetics/*metabolism/pathogenicity Protein Binding Receptors, Tumor Feces/virology Fibroblasts/virology Genetic Fitness/physiology HeLa Cells *Host-Pathogen Interactions Humans Lipopolysaccharides/*metabolism Mice Mice, Virus/metabolism Survival Analysis Viral Plaque Assay Virion/genetics/*metabolism/pathogenicity Virus Attachment Virus Replication},
	issn = {1934-6069 (Electronic) 1931-3128 (Linking)},
	doi = {10.1016/j.chom.2013.12.004},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/24439896},
	author = {Robinson, C. M. and Jesudhasan, P. R. and Pfeiffer, J. K.}
}
