

@article {1786,
	title = {Detection of infectious enteroviruses by an integrated cell culture-PCR procedure},
	journal = {Appl Environ Microbiol},
	volume = {62},
	number = {4},
	year = {1996},
	note = {Reynolds, K A Gerba, C P Pepper, I L eng Comparative Study 1996/04/01 Appl Environ Microbiol. 1996 Apr;62(4):1424-7.},
	pages = {1424-7},
	type = {Journal Article},
	abstract = {Rapid detection of infectious enteroviruses in environmental samples was made possible by utilizing an integrated cell culture-reverse transcriptase PCR approach. By this method, the presence of infectious enterovirus was confirmed within 24 h, compared with > or = 3 days by cell culture alone. The combined methodology eliminated typical problems normally associated with direct reverse transcriptase PCR by increasing the equivalent volume of environmental sample examined and reducing the effects of inhibitory compounds.},
	keywords = {Animals Cell Line Cytopathogenic Effect, Viral Enterovirus/*genetics/*isolation \& purification/pathogenicity Environmental Microbiology Evaluation Studies as Topic Polymerase Chain Reaction/*methods Sewage/virology Virology/*methods},
	issn = {0099-2240 (Print) 0099-2240 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/8919804},
	author = {Reynolds, K. A. and Gerba, C. P. and Pepper, I. L.}
}
