

@article {1767,
	title = {Fecal contamination and diarrheal pathogens on surfaces and in soils among Tanzanian households with and without improved sanitation},
	journal = {Environmental Science and Technology},
	volume = {46},
	number = {11},
	year = {2012},
	note = {Pickering, Amy J Julian, Timothy R Marks, Sara J Mattioli, Mia C Boehm, Alexandria B Schwab, Kellogg J Davis, Jennifer Research Support, Non-U.S. Gov{\textquoteright}t Research Support, U.S. Gov{\textquoteright}t, Non-P.H.S. United States Environmental science \& technology Environ Sci Technol. 2012 Jun 5;46(11):5736-43. doi: 10.1021/es300022c. Epub 2012 May 15.},
	pages = {5736-43},
	type = {Journal Article},
	abstract = {Little is known about the extent or pattern of environmental fecal contamination among households using low-cost, on-site sanitation facilities, or what role environmental contamination plays in the transmission of diarrheal disease. A microbial survey of fecal contamination and selected diarrheal pathogens in soil (n = 200), surface (n = 120), and produce samples (n = 24) was conducted in peri-urban Bagamoyo, Tanzania, among 20 households using private pit latrines. All samples were analyzed for E. coli and enterococci. A subset was analyzed for enterovirus, rotavirus, norovirus GI, norovirus GII, diarrheagenic E. coli, and general and human-specific Bacteroidales fecal markers using molecular methods. Soil collected from the house floor had significantly higher concentrations of E. coli and enterococci than soil collected from the latrine floor. There was no significant difference in fecal indicator bacteria levels between households using pit latrines with a concrete slab (improved sanitation) versus those without a slab. These findings imply that the presence of a concrete slab does not affect the level of fecal contamination in the household environment in this setting. Human Bacteroidales, pathogenic E. coli, enterovirus, and rotavirus genes were detected in soil samples, suggesting that soil should be given more attention as a transmission pathway of diarrheal illness in low-income countries.},
	keywords = {Agricultural/microbiology Diarrhea/*microbiology Enterococcus/growth \& development *Environmental Monitoring Escherichia coli/genetics/growth \& development *Family Characteristics Feces/*microbiology Genes, Bacterial/genetics Humans *Sanitation Sequence Analysis, Colony Count, DNA *Soil Microbiology Surface Properties Tanzania, Microbial Crops},
	issn = {1520-5851 (Electronic) 0013-936X (Linking)},
	doi = {10.1021/es300022c},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/22545817},
	author = {Pickering, A. J. and Julian, T. R. and Marks, S. J. and Mattioli, M. C. and Boehm, A. B. and Schwab, K. J. and Davis, J.}
}
