

@article {1766,
	title = {Techniques for the recovery of enteric viruses from artificially contaminated marine sediments},
	journal = {Igiene e Sanita Pubblica},
	volume = {63},
	number = {4},
	year = {2007},
	note = {Pianetti, Anna Citterio, Barbara Sabatini, Luigia Pierfelici, Lucia Colantoni, Paolo Bruscolini, Francesca eng Evaluation Studies Italy 2007/10/04 09:00 Ig Sanita Pubbl. 2007 Jul-Aug;63(4):419-31.},
	pages = {419-31},
	type = {Journal Article},
	abstract = {<p>Viruses are an important component of aquatic microbial communities and marine sediments may represent an optimal means for their survival. The aim of this study was to evaluate different methods for virus recovery from marine sediments. Three methods were used for virus recovery from artificially contaminated sediments: elution and centrifugation technique, sonication technique, and mechanical disgregation followed by elution and centrifuge technique. The sonication technique obtained the highest virus recovery percentages (94,25\%). Eluent 2 provided more efficient recovery of enteric viruses than eluent 1 presumably due to the presence, in eluent 2, of NANO3, a chaotropic agent that enhances the solubilization of hydrophobic compounds in water. Finally, the authors confirm the importance of searching for viruses in sediments, which protect them from inactivation by biological, chemical and physical factors and allow them to survive longer than in the overlaying water column.</p>
},
	keywords = {Centrifugation, Density Gradient/methods Enterovirus/*isolation \& purification Geologic Sediments/*virology Humans Italy Seawater/*virology Sewage/*virology Sonication Ultrafiltration/methods Virology/*methods},
	issn = {0019-1639 (Print) 0019-1639 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/17912278},
	author = {Pianetti, A. and Citterio, B. and Sabatini, L. and Pierfelici, L. and Colantoni, P. and Bruscolini, F.}
}
