

@article {1759,
	title = {Pre-analytical and analytical procedures for the detection of enteric viruses and enterovirus in water samples},
	journal = {J Virol Methods},
	volume = {184},
	number = {1-2},
	year = {2012},
	note = {Pang, Xiaoli L Lee, Bonita E Pabbaraju, Kanti Gabos, Stephan Craik, Stephen Payment, Pierre Neumann, Norman eng Research Support, Non-U.S. Gov{\textquoteright}t Validation Studies Netherlands 2012/05/29 06:00 J Virol Methods. 2012 Sep;184(1-2):77-83. doi: 10.1016/j.jviromet.2012.05.014. Epub 2012 May 23.},
	pages = {77-83},
	type = {Journal Article},
	abstract = {Practical pre-analytical and analytical procedures were developed and validated for detection of enteric viruses in three water matrices. Both RNA viruses (norovirus, coxsackievirus, echovirus, and rotavirus) and DNA virus (adenovirus 41) were included in the study. The NanoCeram 90mm laminated disc with electropositive filter and procedures of filtration, elution and flocculation were utilized to concentrate known amount of viruses in different water matrices. Real time quantitative PCR was used to evaluate the recovery of virus and cell culture to assess viral infectivity. There was no PCR inhibition using various concentrations and pH of beef extract eluting buffer. A good recovery of the viruses spiked in 10L of deionized water was achieved for serial dilutions of coxsackievirus (41-67\%), echovirus (22-90\%), norovirus (23-44\%) and rotavirus (24-46\%). Relatively lower recovery was observed for adenovirus 41 (24-35\%). There was no significant difference in viral recovery from deionized, tap and river water samples. The infectivity of recovered adenovirus, coxsackievirus and echovirus was demonstrated using in vitro cell culture. The pre-analytical and analytic procedures attained consistent recovery of RNA and DNA viruses both as infectious viral particles and viral genome, provided effective removal of inhibitory substances, achieved reliable reproducibility, and were relatively inexpensive for monitoring viruses in water.},
	keywords = {DNA Viruses/*isolation \& purification Filtration/methods Humans RNA Viruses/*isolation \& purification Real-Time Polymerase Chain Reaction/*methods Specimen Handling/methods Virology/*methods *Water Microbiology},
	issn = {1879-0984 (Electronic) 0166-0934 (Linking)},
	doi = {10.1016/j.jviromet.2012.05.014},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/22633928},
	author = {Pang, X. L. and Lee, B. E. and Pabbaraju, K. and Gabos, S. and Craik, S. and Payment, P. and Neumann, N.}
}
