

@article {1746,
	title = {Pretreatment to avoid positive RT-PCR results with inactivated viruses},
	journal = {J Virol Methods},
	volume = {104},
	number = {2},
	year = {2002},
	note = {Nuanualsuwan, Suphachai Cliver, Dean O eng Research Support, U.S. Gov{\textquoteright}t, Non-P.H.S. Netherlands 2002/06/29 10:00 J Virol Methods. 2002 Jul;104(2):217-25.},
	pages = {217-25},
	type = {Journal Article},
	abstract = {Enteric viruses that are important causes of human disease must often be detected by reverse transcription-polymerase chain reaction (RT-PCR), a method that commonly yields positive results with samples that contain only inactivated virus. This study was intended to develop a pretreatment for samples, so that inactivated viruses would not be detected by the RT-PCR procedure. Model viruses were human hepatitis A virus, vaccine poliovirus 1 and feline calicivirus as a surrogate for the Norwalk-like viruses. Each virus was inactivated (from an initial titer of approximately 10(3) PFU/ml) by ultraviolet light, hypochlorite or heating at 72 degrees C. Inactivated viruses, that were treated with proteinase K and ribonuclease for 30 min at 37 degrees C before RT-PCR, gave a negative result, which is to say that no amplicon was detected after the reaction was completed. This antecedent to the RT-PCR method may be applicable to other types of viruses, to viruses inactivated in other ways and to other molecular methods of virus detection.},
	keywords = {Animal Norovirus/genetics/immunology/isolation \& purification Poliovirus/classification/genetics/immunology/*isolation \& purification Polymerase Chain Reaction/methods *Reverse Transcriptase Polymerase Chain Reaction Vaccines, Animals Calicivirus, Feline/genetics/immunology/*isolation \& purification Cats Cell Line Hepatitis A virus/genetics/immunology/*isolation \& purification Humans Kidney Macaca mulatta Models, Inactivated Viral Vaccines},
	issn = {0166-0934 (Print) 0166-0934 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/12088831},
	author = {Nuanualsuwan, S. and Cliver, D. O.}
}
