

@article {1737,
	title = {Environmental surveillance of poliovirus in sewage water around the introduction period for inactivated polio vaccine in Japan},
	journal = {Applied and environmental microbiology},
	volume = {81},
	number = {5},
	year = {2015},
	note = {Nakamura, Tomofumi Hamasaki, Mitsuhiro Yoshitomi, Hideaki Ishibashi, Tetsuya Yoshiyama, Chiharu Maeda, Eriko Sera, Nobuyuki Yoshida, Hiromu Appl Environ Microbiol. 2015 Mar;81(5):1859-64. doi: 10.1128/AEM.03575-14. Epub 2015 Jan 2.},
	pages = {1859-64},
	type = {Journal Article},
	abstract = {Environmental virus surveillance was conducted at two independent sewage plants from urban and rural areas in the northern prefecture of the Kyushu district, Japan, to trace polioviruses (PVs) within communities. Consequently, 83 PVs were isolated over a 34-month period from April 2010 to January 2013. The frequency of PV isolation at the urban plant was 1.5 times higher than that at the rural plant. Molecular sequence analysis of the viral VP1 gene identified all three serotypes among the PV isolates, with the most prevalent serotype being type 2 (46\%). Nearly all poliovirus isolates exhibited more than one nucleotide mutation from the Sabin vaccine strains. During this study, inactivated poliovirus vaccine (IPV) was introduced for routine immunization on 1 September 2012, replacing the live oral poliovirus vaccine (OPV). Interestingly, the frequency of PV isolation from sewage waters declined before OPV cessation at both sites. Our study highlights the importance of environmental surveillance for the detection of the excretion of PVs from an OPV-immunized population in a highly sensitive manner, during the OPV-to-IPV transition period.},
	issn = {1098-5336 (Electronic) 0099-2240 (Linking)},
	doi = {10.1128/AEM.03575-14},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/25556189},
	author = {Nakamura, T. and Hamasaki, M. and Yoshitomi, H. and Ishibashi, T. and Yoshiyama, C. and Maeda, E. and Sera, N. and Yoshida, H.}
}
