

@article {17163,
	title = {Serotyping, PCR, phage-typing and antibiotic sensitivity testing of Salmonella serovars isolated from urban drinking water supply systems of Nepal},
	journal = {Letters in Applied MicrobiologyLetters in Applied Microbiology},
	volume = {44},
	number = {6},
	year = {2007},
	pages = {588-594},
	abstract = {Abstract Aims:  To study the occurrence and diversity of Salmonella serovars in urban water supply systems of Nepal. Methods and Results:  Occurrence of Salmonella was detected in 42 out of 300 water samples by enrichment culture technique in selenite F broth followed by plating on Salmonella Shigella agar. A total of 54 isolates identified to genus level by standard tests were subsequently confirmed by serotyping, phage typing and PCR detection of virulence genes (inv A and spv C). The predominant serotype was Salmonella Typhimurium, followed by Salm. Typhi, Salm. Paratyphi A and Salmonella Enteritidis. Most of the Salm. Typhi isolates were E1 phage type followed by UVS4, A and UVS1. All isolates of Salm. Paratyphi A and Salm. Enteritidis were an untypable (UT) phage type. The majority of isolates were multi-drug resistant as revealed by Kirby{\textendash}Bauer disc diffusion technique. Ceftriaxone resistant isolates of Salm. Enteritidis indicated the presence of one of the ESBL genes, blaSHV, whereas the genes blaTEM and blaCTX were absent. Conclusions:  The microbiological quality of the urban water supply is poor and indicates possibility of fatal outbreaks of enteric fever and related infections in Nepal. Significance and Impact of the Study:  The present study will be useful in water borne disease control and prevention strategy formulation in Nepal and in the global context.},
	isbn = {0266-8254},
	author = {Bhatta, D.R. and Bangtrakulnonth, A. and Tishyadhigama, P. and Saroj, S.D. and Bandekar, J.R. and Hendriksen, R.S. and Kapadnis, B.P.}
}
