

@article {1709,
	title = {Detection of poliovirus circulation by environmental surveillance in the absence of clinical cases in Israel and the Palestinian authority},
	journal = {J Clin Microbiol},
	volume = {37},
	number = {6},
	year = {1999},
	note = {0095-1137 Journal Article},
	pages = {1670-5},
	type = {Journal Article},
	abstract = {The global eradication of poliomyelitis, believed to be achievable around the year 2000, relies on strategies which include high routine immunization coverage and mass vaccination campaigns, along with continuous monitoring of wild-type virus circulation by using the laboratory-based acute flaccid paralysis (AFP) surveillance. Israel and the Palestinian Authority are located in a geographical region in which poliovirus is still endemic but have been free of poliomyelitis since 1988 as a result of intensive immunization programs and mass vaccination campaigns. To monitor the wild-type virus circulation, environmental surveillance of sewage samples collected monthly from 25 to 30 sites across the country was implemented in 1989 and AFP surveillance began in 1994. The sewage samples were processed in the laboratory with a double-selective tissue culture system, which enabled economical processing of large number of samples. Between 1989 and 1997, 2,294 samples were processed, and wild-type poliovirus was isolated from 17 of them in four clusters, termed "silent outbreaks," in September 1990 (type 3), between May and September 1991 (type 1), between October 1994 and June 1995 (type 1), and in December 1996 (type 1). Fifteen of the 17 positive samples were collected in the Gaza Strip, 1 was collected in the West Bank, and 1 was collected in the Israeli city of Ashdod, located close to the Gaza Strip. The AFP surveillance system failed to detect the circulating wild-type viruses. These findings further emphasize the important role that environmental surveillance can play in monitoring the eradication of polioviruses.},
	keywords = {*Environmental Monitoring Geography Humans Israel Middle East Poliovirus Vaccine, Inactivated Polioviruses/*isolation \& purification Refugees Seasons Sewage/virology Time Factors Urban Health},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=10325305},
	author = {Manor, Y. and Handsher, R. and Halmut, T. and Neuman, M. and Bobrov, A. and Rudich, H. and Vonsover, A. and Shulman, L. and Kew, O. and Mendelson, E.}
}
