

@article {1702,
	title = {Entero- and parechovirus distributions in surface water and probabilities of exposure to these viruses during water recreation},
	journal = {Water Res},
	volume = {75},
	year = {2015},
	note = {Lodder, W J Schijven, J F Rutjes, S A de Roda Husman, A M Teunis, P F M Research Support, Non-U.S. Gov{\textquoteright}t England Water research Water Res. 2015 May 15;75:25-32. doi: 10.1016/j.watres.2015.02.024. Epub 2015 Feb 25.},
	pages = {25-32},
	type = {Journal Article},
	abstract = {Numerous studies have reported quantitative data on viruses in surface waters generated using different methodologies. In the current study, the impact of the use of either cell culture-based or molecular-based methods in quantitative microbial risk assessment was assessed. Previously and newly generated data on the presence of infectious human enteroviruses (HEV) and enterovirus and parechovirus RNA were used to estimate distributions of virus concentrations in surface waters. Because techniques for the detection of infectious human parechoviruses (HPeV) in surface waters were not available, a {\textquoteright}Parallelogram Approach{\textquoteright} was used to estimate their concentrations based on the ratio infectious HEV/HEV RNA. The obtained virus concentrations were then used to estimate the probability of exposure for children during recreation in such virus contaminated surface waters. Human enterovirus cell culture/PCR ratios ranged from 2.3 x 10(-3) to 0.28. This broad range of ratios indicates that care should be taken in assuming a fixed ratio for assessing the risk with PCR based virus concentrations. The probabilities of exposure to both enteroviruses and parechoviruses were calculated, using our Parallelogram Approach for the calculation of infectious parechoviruses. For both viruses it was observed that the detection method significantly influenced the probability of exposure. Based on the calculated culture data, PCR data, and the ingestion volume, it was estimated that the mean probabilities of exposure, of recreating children, to surface water containing viruses were 0.087 (infectious enteroviruses), 0.71 (enterovirus particles), 0.28 (parechovirus particles) and 0.025 (calculated infectious parechoviruses) per recreation event. The mean probabilities of exposure of children recreating in surface water from which drinking water is produced to infectious enteroviruses were estimated for nine locations and varied between 1.5 x 10(-4) - 0.09 per recreation event. In this study, the use of the rotavirus dose response relationship as a surrogate was avoided. Instead, the probabilities of exposure were derived as a function of the distributions of the calculated doses. Our {\textquoteright}Parallelogram Approach{\textquoteright} was used to estimate the unavailable infectious parechovirus concentrations using Monte Carlo simulations, and the exposure assessment carried out showed that virus concentrations present in surface waters could pose a health risk for children and other vulnerable populations.},
	issn = {1879-2448 (Electronic) 0043-1354 (Linking)},
	doi = {10.1016/j.watres.2015.02.024 S0043-1354(15)00097-4 [pii]},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/25746959},
	author = {Lodder, W. J. and Schijven, J. F. and Rutjes, S. A. and A M De Roda Husman and Teunis, P. F.}
}
