

@article {1670,
	title = {Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization},
	journal = {Appl Environ Microbiol},
	volume = {59},
	number = {4},
	year = {1993},
	note = {Kopecka, H Dubrou, S Prevot, J Marechal, J Lopez-Pila, J M eng Comparative Study 1993/04/01 Appl Environ Microbiol. 1993 Apr;59(4):1213-9.},
	pages = {1213-9},
	type = {Journal Article},
	abstract = {Comparison in virus-seeded mineral water of three detection methods for enteroviruses, direct hybridization, cell culture, and reverse transcription into cDNA followed by polymerase chain reaction and hybridization, showed that the last procedure was 10 to 1,000 times more sensitive than detection by cell culture and 10(5) to 10(7) times more sensitive than direct hybridization. The presence of naturally occurring enteroviruses was also demonstrated in activated sludge and in concentrated and non-concentrated surface water samples by reverse transcription-polymerase chain reaction-hybridization. However, in activated sludge and in concentrated surface waters, enzymatic amplification was sometimes inhibited by contaminants.},
	keywords = {Animals Base Sequence Cercopithecus aethiops DNA, Single-Stranded Enterovirus/growth \& development/*isolation \& purification HeLa Cells Humans Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction RNA, Viral/analysis RNA-Directed DNA Polymerase/metabolism Sensitivity and Specificity *Sewage Vero Cells Virology/*methods Virus Cultivation *Water Microbiology},
	issn = {0099-2240 (Print) 0099-2240 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/7683857},
	author = {Kopecka, H. and Dubrou, S. and Prevot, J. and Marechal, J. and Lopez-Pila, J. M.}
}
