

@article {1662,
	title = {Poliovirus serotype-specific VP1 sequencing primers},
	journal = {J Virol Methods},
	volume = {174},
	number = {1-2},
	year = {2011},
	note = {Kilpatrick, David R Iber, Jane C Chen, Qi Ching, Karen Yang, Su-Ju De, Lina Mandelbaum, Mark D Emery, Brian Campagnoli, Ray Burns, Cara C Kew, Olen Evaluation Studies Netherlands Journal of virological methods J Virol Methods. 2011 Jun;174(1-2):128-30. doi: 10.1016/j.jviromet.2011.03.020. Epub 2011 Apr 2.},
	pages = {128-30},
	type = {Journal Article},
	abstract = {The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance. To provide detailed molecular epidemiologic information, poliovirus isolates are further characterized by sequencing the ~900-nucleotide region encoding the major capsid protein, VP1. It is difficult to obtain quality sequence information when clinical or environmental samples contain poliovirus mixtures. As an alternative to conventional methods for resolving poliovirus mixtures, sets of serotype-specific primers were developed for amplifying and sequencing the VP1 regions of individual components of mixed populations of vaccine-vaccine, vaccine-wild, and wild-wild polioviruses.},
	keywords = {Capsid Proteins/*genetics DNA Primers/*genetics Humans Poliovirus/*classification/*genetics/isolation \& purification Polymerase Chain Reaction/*methods Sensitivity and Specificity *Sequence Analysis, DNA Virology/methods},
	issn = {1879-0984 (Electronic) 0166-0934 (Linking)},
	doi = {10.1016/j.jviromet.2011.03.020 S0166-0934(11)00111-X [pii]},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/21440569},
	author = {Kilpatrick, D. R. and Iber, J. C. and Chen, Q. and Ching, K. and Yang, S. J. and De, L. and Mandelbaum, M. D. and Emery, B. and Campagnoli, R. and Burns, C. C. and Kew, O.}
}
