

@article {16600,
	title = {Early exposure of infants to {GI} nematodes induces {Th}2 dominant immune responses which are unaffected by periodic anthelminthic treatment},
	journal = {PLoS Negl Trop Dis},
	volume = {3},
	number = {5},
	year = {2009},
	pages = {e433},
	abstract = {We have previously shown a reduction in anaemia and wasting malnutrition in infants {\textless}3 years old in Pemba Island, Zanzibar, following repeated anthelminthic treatment for the endemic gastrointestinal (GI) nematodes Ascaris lumbricoides, hookworm and Trichuris trichiura. In view of the low intensity of worm infections in this age group, this was unexpected, and it was proposed that immune responses to the worms rather than their direct effects may play a significant role in morbidity in infants and that anthelminthic treatment may alleviate such effects. Therefore, the primary aims of this study were to characterise the immune response to initial/early GI nematode infections in infants and the effects of anthelminthic treatment on such immune responses. The frequency and levels of Th1/Th2 cytokines (IL-5, IL-13, IFN-gamma and IL-10) induced by the worms were evaluated in 666 infants aged 6-24 months using the Whole Blood Assay. Ascaris and hookworm antigens induced predominantly Th2 cytokine responses, and levels of IL-5 and IL-13 were significantly correlated. The frequencies and levels of responses were higher for both Ascaris positive and hookworm positive infants compared with worm negative individuals, but very few infants made Trichuris-specific cytokine responses. Infants treated every 3 months with mebendazole showed a significantly lower prevalence of infection compared with placebo-treated controls at one year following baseline. At follow-up, cytokine responses to Ascaris and hookworm antigens, which remained Th2 biased, were increased compared with baseline but were not significantly affected by treatment. However, blood eosinophil levels, which were elevated in worm-infected children, were significantly lower in treated children. Thus the effect of deworming in this age group on anaemia and wasting malnutrition, which were replicated in this study, could not be explained by modification of cytokine responses but may be related to eosinophil function.},
	keywords = {Ancylostomatoidea/immunology/physiology, Animals, Anthelmintics/*therapeutic use, Ascaris lumbricoides/immunology/pathogenicity, Enzyme-Linked Immunosorbent Assay, Female, Gastrointestinal Diseases/drug therapy/*immunology/*parasitology, Humans, Infant, Interferon-gamma/metabolism, Interleukin-10/metabolism, Interleukin-13/metabolism, Interleukin-5/metabolism, Male, Nematode Infections/drug therapy/*immunology/*physiopathology, Trichuris/immunology/physiology},
	issn = {1935-2735 (Electronic) 1935-2727 (Linking)},
	doi = {10.1371/journal.pntd.0000433},
	author = {Wright, V. J. and Ame, S. M. and Haji, H. S. and Weir, R. E. and Goodman, D. and Pritchard, D. I. and Mohamed, M. R. and Haji, H. J. and Tielsch, J. M. and Stoltzfus, R. J. and Bickle, Q. D.}
}
