@article {16047, title = {Molecular Characterization of Leptospira spp. in Environmental Samples from North-Eastern Malaysia Revealed a Pathogenic Strain, Leptospira alstonii}, journal = {J Trop MedJ Trop MedJ Trop Med}, volume = {2016}, year = {2016}, note = {Azali, Muhammad Azharuddin
Yean Yean, Chan
Harun, Azian
Aminuddin Baki, Nurul Najian
Ismail, Nabilah
Egypt
J Trop Med. 2016;2016:2060241. doi: 10.1155/2016/2060241. Epub 2016 Apr 3.}, pages = {2060241}, edition = {2016/04/30}, abstract = {The presence of pathogenic Leptospira spp. in the environment poses threats to human health. The aim of this study was to detect and characterize Leptospira spp. from environmental samples. A total of 144 samples comprised of 72 soil and 72 water samples were collected from markets and recreational areas in a north-eastern state in Malaysia. Samples were cultured on Ellinghausen and McCullough modified by Johnson and Harris media. Leptospires were positive in 22.9\% (n = 33) of the isolates. Based on partial sequences of 16S rRNA, a pathogenic leptospire, Leptospira alstonii (n = 1/33), was identified in 3\% of the isolates followed by intermediate leptospire (L. wolffii, n = 1/33, and L. licerasiae, n = 7/33) and nonpathogenic leptospire, L. meyeri (n = 22/33) in 24.2\% and 66.7\%, respectively. This study demonstrates the presence of a clinically significant pathogenic L. alstonii in the environments which could pose health risks to the occupants and visitors.}, isbn = {1687-9686 (Print)
1687-9686 (Linking)}, author = {Azali, M. A. and Yean Yean, C. and Harun, A. and Aminuddin Baki, N. N. and Ismail, N.} }