

@article {1579,
	title = {Evaluation of positively charged alumina nanofibre cartridge filters for the primary concentration of noroviruses, adenoviruses and male-specific coliphages from seawater},
	journal = {Journal of Applied Microbiology},
	volume = {109},
	number = {2},
	year = {2010},
	note = {Gibbons, C D Rodriguez, R A Tallon, L Sobsey, M D England J Appl Microbiol. 2010 Aug;109(2):635-41. doi: 10.1111/j.1365-2672.2010.04691.x. Epub 2010 Feb 19.},
	pages = {635-41},
	type = {Journal Article},
	abstract = {<p>AIM: To evaluate the electropositive, alumina nanofibre (NanoCeram) cartridge filter as a primary concentration method for recovering adenovirus, norovirus and male-specific coliphages from natural seawater. METHODS AND RESULTS: Viruses were concentrated from 40 l of natural seawater using a NanoCeram cartridge filter and eluted from the filter either by soaking the filter in eluent or by recirculating the eluent continuously through the filter using a peristaltic pump. The elution solution consisted of 3\% beef extract and 0.1 mol l(-1) of glycine. The method using a peristaltic pump was more effective in removing the viruses from the filter. High recoveries of norovirus and male-specific coliphages (\&gt;96\%) but not adenovirus (\&lt;3\%) were observed from seawater. High adsorption to the filter was observed for adenovirus and male-specific coliphages (\&gt;98\%). The adsorption and recovery of adenovirus and male-specific coliphages were also determined for fresh finished water and source water. CONCLUSION: The NanoCeram cartridge filter was an effective primary concentration method for the concentration of norovirus and male-specific coliphages from natural seawater, but not for adenovirus, in spite of the high adsorption of adenovirus to the filter. SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates that NanoCeram cartridge filter is an effective primary method for concentrating noroviruses and male-specific coliphages from seawater, thereby simplifying collection and processing of water samples for virus recovery.</p>
},
	keywords = {Adenoviridae/*isolation \& purification Adsorption Aluminum Oxide/chemistry Coliphages/*isolation \& purification Filtration/instrumentation Fresh Water/virology Nanostructures/chemistry Norovirus/*isolation \& purification Seawater/*virology Water Microbiol},
	issn = {1365-2672 (Electronic) 1364-5072 (Linking)},
	doi = {10.1111/j.1365-2672.2010.04691.x},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/20202019},
	author = {Gibbons, C. D. and Rodriguez, R. A. and Tallon, L. and Sobsey, M. D.}
}
