@article {15492, title = {Immune responses during human schistosomiasis mansoni. XVI. Idiotypic differences in antibody preparations from patients with different clinical forms of infection}, journal = {J Immunol}, volume = {142}, number = {7}, year = {1989}, note = {Montesano, M A
Lima, M S
Correa-Oliveira, R
Gazzinelli, G
Colley, D G
eng
Research Support, Non-U.S. Gov{\textquoteright}t
Research Support, U.S. Gov{\textquoteright}t, Non-P.H.S.
Research Support, U.S. Gov{\textquoteright}t, P.H.S.
Baltimore, Md. : 1950
1989/04/01
J Immunol. 1989 Apr 1;142(7):2501-6.}, month = {Apr 1}, pages = {2501-6}, abstract = {Antibodies were purified from pooled sera from patients with different clinical forms of schistosomiasis mansoni on immunoaffinity columns of schistosome soluble egg Ag (SEA). As previously reported, T lymphocytes in PBMC preparations from schistosomiasis patients (but not control subjects who have never been infected) proliferate when cultured in the presence of certain of these anti-SEA purified antibodies. We now show that PBMC from most patients with chronic schistosomiasis, regardless of the clinical form of their infection, respond to anti-SEA antibodies from sera of asymptomatic (intestinal) or hepatointestinal patients. In stark contrast, none responds to anti-SEA antibodies purified from sera of acute or hepatosplenic patients. All of these multiclonal anti-SEA antibody preparations were active in anti-SEA ELISA assays and gave comparable patterns of reactivity with SEA upon immunoblotting analysis. Immunization of rabbits with some of these anti-SEA antibody preparations, followed by absorption of the rabbit antisera on absorbents of normal Ig, produced specific anti-Id reagents. Use of these reagents in competitive ELISA systems demonstrated that the Id in stimulatory and nonstimulatory anti-SEA antibody preparations differ with regard to the proportion of the serologically defined Id expressed by each. It appears possible to screen patients{\textquoteright} plasmas for the presence of shared Id by use of suitable Id/anti-Id competitive ELISA assays. Taken together these data indicate that only certain Id-positive preparations are stimulatory to patients{\textquoteright} PBMC, and the expression of these T cell stimulatory, immunoregulatory Id on anti-SEA antibodies correlates with the clinical form of a patient{\textquoteright}s infection.}, keywords = {Acute Disease, Animals, Antibodies, Anti-Idiotypic/biosynthesis, Antibodies, Helminth/administration \& dosage/*biosynthesis/isolation \&, Antibody Specificity, Antigens, Helminth/immunology, Binding, Competitive, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin Idiotypes/analysis/*biosynthesis, Immunosorbent Techniques, Liver Diseases, Parasitic/immunology, Lymphocyte Activation, Ovum/immunology, purification, Rabbits, Schistosomiasis mansoni/*immunology, Splenic Diseases/immunology/parasitology}, isbn = {0022-1767 (Print)
0022-1767 (Linking)}, author = {Montesano, M. A. and Lima, M. S. and Correa-Oliveira, R. and Gazzinelli, G. and Colley, D. G.} }