@article {15471, title = {An IL-13 promoter polymorphism associated with liver fibrosis in patients with Schistosoma japonicum}, journal = {PLoS One}, volume = {10}, number = {8}, year = {2015}, note = {R01HL112088/HL/NHLBI NIH HHS/United States
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov{\textquoteright}t
United States}, pages = {e0135360}, abstract = {The aim of this study was to determine whether two polymorphisms in the gene encoding IL13 previously associated with Schistosoma hematobium (S. hematobium) and S. mansoni infection are associated with S. japonicum infection. Single nucleotide polymorphisms (SNPs) rs1800925 (IL13/-1112C>T) and rs20541 (IL13R130Q) were genotyped in 947 unrelated individuals (307 chronically infected, 339 late-stage with liver fibrosis, 301 uninfected controls) from a schistosomiasis-endemic area of Hubei province in China. Regression models were used to evaluate allelic and haplotypic associations with chronic and late-stage schistosomiasis adjusted for non-genetic covariates. Expression of IL-13 was measured in S. japonicun-infected liver fibrosis tissue and normal liver tissue from uninfected controls by immunohistochemistry (IHC). The role of rs1800925 in IL-13 transcription was further determined by Luciferase report assay using the recombinant PGL4.17-rs180092 plasmid. We found SNP rs1800925T was associated with late-stage schistosomiasis caused by S. japonicum but not chronic schistosomiasis (OR = 1.39, 95\%CI = 1.02-1.91, p = 0.03) and uninfected controls (OR = 1.49, 95\%CI = 1.03-2.13, p = 0.03). Moreover, the haplotype rs1800925T-rs20541C increased the risk of disease progression to late-stage schistosomiasis (OR = 1.46, p = 0.035), whereas haplotype rs1800925C-rs20541A showed a protective role against development of late-stage schistosomiasis (F = 0.188, OR = 0.61, p = 0.002). Furthermore, S. japonicum-induced fibrotic liver tissue had higher IL13 expression than normal liver tissue. Plasmid PGL4.17-rs1800925T showed a stronger relative luciferase activity than Plasmid PGL4.17-rs1800925C in 293FT, QSG-7701 and HL-7702 cell lines. In conclusion, the functional IL13 polymorphism, rs1800925T, previously associated with risk of schistosomiasis, also contributes to risk of late-stage schistosomiasis caused by S. japonicum.}, keywords = {*Polymorphism, Single Nucleotide, Adult, Animals, Case-Control Studies, Disease Progression, Female, Gene expression, Haplotypes, Host-Parasite Interactions, Humans, Interleukin-13/*genetics/metabolism, Liver Cirrhosis/complications/*genetics/parasitology/pathology, Liver/*metabolism/parasitology/pathology, Male, Middle Aged, Odds Ratio, Promoter Regions, Genetic, Risk, Schistosoma japonicum/*pathogenicity/physiology, Schistosomiasis japonica/complications/*genetics/parasitology/pathology, Severity of Illness Index}, isbn = {1932-6203 (Electronic)
1932-6203 (Linking)}, author = {Long, X. and Chen, Q. and Zhao, J. and Rafaels, N. and Mathias, P. and Liang, H. and Potee, J. and Campbell, M. and Zhang, B. and Gao, L. and Georas, S. N. and Vercelli, D. and Beaty, T. H. and Ruczinski, I. and Mathias, R. and Barnes, K. C. and Chen, X.} }