

@article {15373,
	title = {Biophysical and formulation studies of the Schistosoma mansoni TSP-2 extracellular domain recombinant protein, a lead vaccine candidate antigen for intestinal schistosomiasis},
	journal = {Human Vaccines and Immunotherapeutics},
	volume = {9},
	number = {11},
	year = {2013},
	note = {Journal Article<br/>United States},
	month = {Nov},
	pages = {2351-61},
	abstract = {A candidate vaccine to prevent human schistosomiasis is under development. The vaccine is comprised of a recombinant 9 kDa antigen protein corresponding to the large extracellular domain of a tetraspanin surface antigen protein of Schistosoma mansoni, Sm-TSP-2. Here, we describe the biophysical profile of the purified, recombinant Sm-TSP-2 produced in the yeast PichiaPink, which in preclinical studies in mice was shown to be an effective vaccine against intestinal schistosomiasis. Biophysical techniques including circular dichroism, intrinsic and extrinsic fluorescence and light scattering were employed to generate an empirical phase diagram, a color based map of the physical stability of the vaccine antigen over a wide range of temperatures and pH. From these studies a pH range of 6.0-8.0 was determined to be optimal for maintaining the stability and conformation of the protein at temperatures up to 25 degrees C. Sorbitol, sucrose and trehalose were selected as excipients that prevented physical degradation during storage. The studies described here provide guidance for maximizing the stability of soluble recombinant Sm-TSP-2 in preparation of its further development as a vaccine.},
	keywords = {Animals, Antigens, Helminth/*chemistry/genetics/isolation \& purification, Biophysical Phenomena, Circular Dichroism, Drug Compounding, Drug Stability, Excipients/pharmacology, Fluorometry, Helminth Proteins/*chemistry/genetics/isolation \& purification, Humans, Hydrogen-Ion Concentration, Mice, Pichia/genetics, Protein Conformation, Protein Stability, Recombinant Proteins/chemistry/genetics/isolation \& purification, Schistosomiasis mansoni/immunology/*prevention \& control, Temperature, Tetraspanins/*chemistry/genetics/isolation \& purification, Vaccines/*chemistry/*immunology/isolation \& purification},
	isbn = {2164-554X (Electronic)<br/>2164-5515 (Linking)},
	author = {Cheng, W. and Curti, E. and Rezende, W. C. and Kwityn, C. and Zhan, B. and Gillespie, P. and Plieskatt, J. and Joshi, S. B. and Volkin, D. B. and Hotez, P. J. and Middaugh, C. R. and Bottazzi, M. E.}
}
