@article {15350, title = {Diagnosis of human schistosomiasis by detection of circulating cathodic antigen with a monoclonal antibody}, journal = {J Infect Dis}, volume = {164}, number = {5}, year = {1991}, note = {Barsoum, I S
Kamal, K A
Bassily, S
Deelder, A M
Colley, D G
eng
Research Support, U.S. Gov{\textquoteright}t, Non-P.H.S.
1991/11/01
J Infect Dis. 1991 Nov;164(5):1010-3.}, month = {Nov}, pages = {1010-3}, abstract = {Monoclonal antibody (MAb) 5H11 reacted with repeating epitopes on Schistosoma mansoni circulating cathodic antigen (CCA) and detected CCA in sera of Egyptian S. mansoni-infected patients. MAb 5H11 was both capture and biotinylated detection antibody in a sandwich ELISA of trichloroacetic acid-pretreated serum samples. Sera of patients with 7-500 eggs/g of stool were positive by MAb 5H11-CCA sandwich ELISA. Stool egg counts and CCA serum levels correlated (r = .52), and CCA levels decreased by 4 weeks after praziquantel treatment in patients with pretreatment egg counts of greater than or equal to 50/g of stool (P less than .05). Sera of Schistosoma haematobium-infected patients, uninfected individuals, and most patients with other helminths were negative in this assay. The MAb 5H11-CCA sandwich ELISA appears sensitive and specific for immunodetection of active schistosomiasis mansoni and useful for monitoring its chemotherapy.}, keywords = {*Antibodies, Monoclonal/immunology, Adolescent, Adult, Animals, Antibody Specificity, Antigens, Helminth/*blood/immunology, Child, Enzyme-Linked Immunosorbent Assay, Feces/parasitology, Glycoproteins/*blood, Helminth Proteins/*blood, Humans, Male, Middle Aged, Parasite Egg Count, Praziquantel/therapeutic use, Schistosoma mansoni/*immunology/isolation \& purification, Schistosomiasis mansoni/*diagnosis/drug therapy}, isbn = {0022-1899 (Print)
0022-1899 (Linking)}, author = {Barsoum, I. S. and Kamal, K. A. and Bassily, S. and Deelder, A. M. and Colley, D. G.} }