

@article {15130,
	title = {Molecular identification of {Spirometra} spp. ({Cestoda}: {Diphyllobothriidae}) in some wild animals from {Brazil}.},
	journal = {Parasitology international},
	volume = {65},
	number = {5 Pt A},
	year = {2016},
	month = {oct},
	pages = {428{\textendash}431},
	abstract = {Species of the genus Spirometra are diphyllobothriid tapeworms with complex life cycles and are involved in human sparganosis, a neglected disease that affects individuals worldwide. Although some species were reported in wild felids and human cases of sparganosis were described in Brazil, the biology and taxonomy of these parasites are poorly understood. In the present study, samples of diphyllobothriids (eggs and/or proglottids) obtained from the stools of wild carnivores (Leopardus pardalis and Lycalopex vetulus) and plerocercoid larvae found in a snake (Crotalus durissus) from Brazil were analysed by amplifying a fragment of the gene cytochrome c oxidase subunit 1 (cox 1). The DNA sequences obtained here for the first time from the Spirometra spp. from Brazil were used to evaluate the phylogenetic relationships with other species. Molecular data identified two species in the Brazilian samples (evolutionary divergence of 17.8-19.2\%). The species were identified as Spirometra sp. 1, found in Le. pardalis, and Spirometra sp. 2 found in Ly. vetulus and C. durissus, and they differed from Asian isolates of Spirometra erinaceieuropaei (17.5-20.2\% and 12.2-15.6\%, respectively), a species previously considered to be distributed worldwide. Moreover, Spirometra sp. 1 is genetically distinct from Sparganum proliferum from Venezuela (19.6-20.4\%), while Spirometra sp. 2 is more closely related with the Venezuelan species (6.1-7.0\%). Sequences of Spirometra sp. 2 revealed that it is conspecific with the Argentinean isolate of Spirometra found in Lycalopex gymnocercus (1.9-2.2\%). Taxonomic and phylogenetic aspects related to New World species of Spirometra are briefly discussed.},
	keywords = {Brazil, Cestodes, Molecular identification, Spirometra},
	issn = {1873-0329},
	doi = {10.1016/j.parint.2016.05.014},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/27235572},
	author = {Almeida, Greg{\'o}rio Guilherme and Coscarelli, Daniel and Melo, Maria Norma and Melo, Alan Lane and Pinto, Hudson Alves}
}
