

@article {15012,
	title = {Poliovirus serotype-specific {VP}1 sequencing primers},
	journal = {J Virol Methods},
	volume = {174},
	year = {2011},
	month = {jun},
	pages = {128{\textendash}30},
	abstract = {The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance. To provide detailed molecular epidemiologic information, poliovirus isolates are further characterized by sequencing the {\textbackslash}textasciitilde900-nucleotide region encoding the major capsid protein, VP1. It is difficult to obtain quality sequence information when clinical or environmental samples contain poliovirus mixtures. As an alternative to conventional methods for resolving poliovirus mixtures, sets of serotype-specific primers were developed for amplifying and sequencing the VP1 regions of individual components of mixed populations of vaccine-vaccine, vaccine-wild, and wild-wild polioviruses.},
	keywords = {*Sequence Analysis, Capsid Proteins/*genetics, DNA, DNA Primers/*genetics, Humans, Poliovirus/*classification/*genetics/isolation \& purification, Polymerase Chain Reaction/*methods, Sensitivity and Specificity, Virology/methods},
	issn = {1879-0984 (Electronic) 0166-0934 (Linking)},
	doi = {10.1016/j.jviromet.2011.03.020 S0166-0934(11)00111-X [pii]},
	author = {Kilpatrick, D. R. and Iber, J. C. and Chen, Q. and Ching, K. and Yang, S. J. and De, L. and Mandelbaum, M. D. and Emery, B. and Campagnoli, R. and Burns, C. C. and Kew, O.}
}
