

@article {14967,
	title = {The complete genome of klassevirus - a novel picornavirus in pediatric stool},
	journal = {Virol J},
	volume = {6},
	year = {2009},
	pages = {82},
	abstract = {BACKGROUND: Diarrhea kills 2 million children worldwide each year, yet an etiological agent is not found in approximately 30-50\% of cases. Picornaviral genera such as enterovirus, kobuvirus, cosavirus, parechovirus, hepatovirus, teschovirus, and cardiovirus have all been found in human and animal diarrhea. Modern technologies, especially deep sequencing, allow rapid, high-throughput screening of clinical samples such as stool for new infectious agents associated with human disease. RESULTS: A pool of 141 pediatric gastroenteritis samples that were previously found to be negative for known diarrheal viruses was subjected to pyrosequencing. From a total of 937,935 sequence reads, a collection of 849 reads distantly related to Aichi virus were assembled and found to comprise 75\% of a novel picornavirus genome. The complete genome was subsequently cloned and found to share 52.3\% nucleotide pairwise identity and 38.9\% amino acid identity to Aichi virus. The low level of sequence identity suggests a novel picornavirus genus which we have designated klassevirus. Blinded screening of 751 stool specimens from both symptomatic and asymptomatic individuals revealed a second positive case of klassevirus infection, which was subsequently found to be from the index case{\textquoteright}s 11-month old twin. CONCLUSION: We report the discovery of human klassevirus 1, a member of a novel picornavirus genus, in stool from two infants from Northern California. Further characterization and epidemiological studies will be required to establish whether klasseviruses are significant causes of human infection.},
	keywords = {*Genome, *Sequence Analysis, Amino Acid, California, DNA, Feces/*virology, Gastroenteritis/*virology, Humans, Molecular Sequence Data, Nucleic Acid, Picornaviridae Infections/*virology, Picornaviridae/*genetics/*isolation \& purification, RNA, Sequence Homology, Viral, Viral/*genetics},
	issn = {1743-422X (Electronic) 1743-422X (Linking)},
	doi = {10.1186/1743-422X-6-82},
	author = {Greninger, A. L. and Runckel, C. and Chiu, C. Y. and T Haggerty and Parsonnet, J. and Ganem, D. and DeRisi, J. L.}
}
