

@article {1495,
	title = {Sample preparation prior to molecular amplification: complexities and opportunities},
	journal = {Curr Opin Virol},
	volume = {4},
	year = {2014},
	note = {Butot, Sophie Zuber, Sophie Baert, Leen eng Review Netherlands 2014/01/21 06:00 Curr Opin Virol. 2014 Feb;4:66-70. doi: 10.1016/j.coviro.2013.12.004. Epub 2014 Jan 16.},
	pages = {66-70},
	type = {Journal Article},
	abstract = {Molecular amplification using Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) is currently considered as the gold standard to detect enteric human pathogenic viruses such as norovirus and hepatitis A virus in food and water. However, the molecular-based detection requires an adequate sampling strategy and a sample preparation specific for viruses. Sampling for enteric human viruses in water and food should not necessarily follow bacterial sampling plans. The development of a reference detection method including sample preparation as proposed in ISO/TS 15216 represents a milestone to facilitate the evaluation of the performance and eventually validation of future virus detection methods. The potential viral infectivity linked to a positive PCR result is a remaining issue and pretreatments allowing the differentiation of infectious viruses would be useful for future risk assessments.},
	keywords = {Food Microbiology Humans Real-Time Polymerase Chain Reaction/*methods Specimen Handling/*methods Virology/*methods Viruses/*isolation \& purification Water Microbiology},
	issn = {1879-6265 (Electronic)},
	doi = {10.1016/j.coviro.2013.12.004},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/24441295},
	author = {Butot, S. and Zuber, S. and Baert, L.}
}
