

@article {14730,
	title = {Development of a virus concentration method and its application to detection of enterovirus and norwalk virus from coastal seawater},
	journal = {Appl Environ Microbiol},
	volume = {68},
	year = {2002},
	month = {mar},
	pages = {1033{\textendash}9},
	abstract = {We developed a new procedure for concentration of enteric viruses from water using a negatively charged membrane. Rinsing the membrane with 0.5 mM H(2)SO(4) (pH 3.0) in order to elute cations prior to viral elution with 1 mM NaOH (pH 10.5) promoted poliovirus recovery yields from 33 to 95\% when applied to pure water and 38 to 89\% when applied to natural seawater from Tokyo Bay, Japan, respectively. This method showed average recovery yields of spiked poliovirus of 62\% (n = 8) from 1 liter of artificial seawater. This method showed higher recovery yields ({\textgreater}61\%) than that of the conventional method using positively charged membrane (6\%) when applied to seawater. This method is also free from beef extract elution, which has an inhibitory effect in the subsequent viral genome detection by reverse transcription-PCR. Naturally occurring Norwalk viruses from 2 liters of Tokyo Bay water in winter and infectious enteroviruses from 2 liters of recreational coastal seawater in summer were detected by using this viral concentration method.},
	keywords = {*Seawater, *Water Microbiology, Enterovirus/*isolation \& purification, Norwalk virus/*isolation \& purification, Virology/methods},
	issn = {0099-2240 (Print) 0099-2240 (Linking)},
	author = {Katayama, H. and Shimasaki, A. and Ohgaki, S.}
}
