

@article {14710,
	title = {Development of a novel filter cartridge system with electropositive granule media to concentrate viruses from large volumes of natural surface water},
	journal = {Environ Sci Technol},
	volume = {48},
	year = {2014},
	month = {jun},
	pages = {6947{\textendash}56},
	abstract = {Exposure to various infectious viruses in environmental drinking water can constitute a public health risk. However, it is difficult to detect viruses in water due to their low concentration. In this study, we have developed a novel filter cartridge system containing electropositive granule media (EGM). Viruses present in large volumes of environmental samples were adsorbed onto the EGM, and then recovered by elution and poly(ethylene glycol) (PEG) concentration. To evaluate the system{\textquoteright}s efficiency in viral recovery, poliovirus (PV-1), a surrogate for enteric viruses, was used to artificially contaminate river water samples which were then assayed by quantitative real-time PCR. To optimize the concentration procedure, the eluent type, water flow rate and properties (e.g., pH, bacterial, and viral loads), were evaluated. The highest virus recovery was obtained by pumping river water at a flow rate of 300 mL/min and then pushing 3 L of an eluent containing 3x broth [1.5\% (w/v) NaCl, 3\% (w/v) tryptone, 1.5\% (w/v) beef powder] with 0.05 mol/L glycine through the filter. Using this procedure, the recovery efficiencies of PV-1 from 10 to 100 L of spiked river water were up to 99\%. In addition, this method is virus load and pH dependent. Virus recovery was maximal at a load of between 10(3.5) and 10(5.5) TCID50 and a pH ranging from 5 to 7. The bacterial load in the water has no effect on virus recovery. Different types of viruses and surface water were tested to validate the system{\textquoteright}s applicability. Results revealed that the EGM filter cartridge was able to concentrate PV-1, human adenoviruses (HAdVs) and noroviruses (HuNoVs) with high efficiency from river, lake, and reservoir water. Furthermore, it showed more efficient recovery than glass wool and 1MDS filters. These data suggest that this system provides rapid and efficient virus recovery from a large volume of natural surface water and, as such, could be a useful tool in revealing the presence of viruses in surface water.},
	keywords = {*Water Microbiology, Adsorption, Aluminum Oxide/chemistry, Animals, Cell Line, Chemical Precipitation, Electrodes, Escherichia coli/growth \& development, Filtration/*instrumentation/*methods, Humans, Hydrogen-Ion Concentration, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Rheology, Rivers/virology, Viruses/genetics/*isolation \& purification, Water quality},
	issn = {1520-5851 (Electronic) 0013-936X (Linking)},
	doi = {10.1021/es501415m},
	author = {Jin, M. and Guo, X. and Wang, X. W. and Yang, D. and Shen, Z. Q. and Qiu, Z. G. and Chen, Z. L. and Li, J. W.}
}
