

@article {14700,
	title = {Development of real-time {PCR} to detect oral vaccine-like poliovirus and its application to environmental surveillance},
	journal = {J Virol Methods},
	volume = {195},
	year = {2014},
	month = {jan},
	pages = {148{\textendash}55},
	abstract = {In order to perform environmental surveillance to track oral poliovirus vaccine-like poliovirus sensitively and conveniently, real-time PCR was developed and applied to a raw sewage concentrate. The real-time PCR method detected 0.01-0.1 TCID50 of 3 serotypes of Sabin strain specifically. The method also detected the corresponding serotypes of oral poliovirus vaccine-like poliovirus specifically, but detected neither wild poliovirus, except Mahoney for type 1 and Saukett for type 3, nor other enteric viruses, as far as examined. When real-time PCR was applied to environmental surveillance, the overall agreement rates between real-time PCR and the cell culture were 83.3\% for all serotypes. Since real-time PCR has the advantages of rapid detection of viruses and minimum requirement of sampling volume as compared with ordinary cell culture, it is suitable to monitor oral poliovirus vaccine-like poliovirus in the environment, especially in areas where an oral vaccine is being replaced by an inactivated vaccine.},
	keywords = {Environmental Monitoring/*methods, Environmental Pollutants/*isolation \& purification, Humans, Oral/*isolation \& purification, Poliovirus Vaccine, Poliovirus/classification/genetics/*isolation \& purification, Real-Time Polymerase Chain Reaction/*methods, Sewage/virology},
	issn = {1879-0984 (Electronic) 0166-0934 (Linking)},
	doi = {10.1016/j.jviromet.2013.10.004},
	author = {Iwai-Itamochi, M. and Yoshida, H. and Obara-Nagoya, M. and Horimoto, E. and Kurata, T. and Takizawa, T.}
}
